Mannucci A, Sullivan K M, Ivanov P L, Gill P
Istituto di Medicina Legale, Universita' di Genova, Italy.
Int J Legal Med. 1994;106(4):190-3. doi: 10.1007/BF01371335.
Gender identification of forensic samples was determined by amplifying a segment of the X-Y homologous gene amelogenin. Using a single pair of primers spanning part of the first intron, 106 bp and 112 bp PCR products were generated from the X and Y homologues respectively, which were then resolved by agarose gel electrophoresis. This test enabled as little as 20 pg of DNA from severely degraded bones to be amplified and typed in a single tube reaction. Furthermore, using dye-labelled primers, it was possible to quantitate, by automated fluorescence detection, the relative yields of X and Y-specific PCR products generated from mixtures of male and female DNA. The versatility of this sex test was further demonstrated by co-amplifying with the HLA-DQA1 Amplitype kit in a combined gender/identity DNA test.
法医样本的性别鉴定是通过扩增一段X-Y同源基因牙釉蛋白来确定的。使用一对跨越第一个内含子部分的引物，分别从X和Y同源物中产生106 bp和112 bp的PCR产物，然后通过琼脂糖凝胶电泳进行分离。该测试能够在单管反应中扩增并分型来自严重降解骨骼的低至20 pg的DNA。此外，使用染料标记的引物，可以通过自动荧光检测对从男性和女性DNA混合物中产生的X和Y特异性PCR产物的相对产量进行定量。通过在性别/身份联合DNA测试中与HLA-DQA1 Amplitype试剂盒共同扩增，进一步证明了这种性别测试的通用性。
