基于序列的德巴利酵母属物种鉴定
Sequence-based identification of species belonging to the genus Debaryomyces.
作者信息
Martorell Patricia, Fernández-Espinar M Teresa, Querol Amparo
机构信息
Departamento de Biotecnología de los Alimentos, Instituto de Agroquímica y Tecnología de Alimentos CSIC, P.O. Box 73, E-46100 Burjassot, València, Spain.
出版信息
FEMS Yeast Res. 2005 Dec;5(12):1157-65. doi: 10.1016/j.femsyr.2005.05.002. Epub 2005 Jun 29.
In the present study we assessed the identification by sequence analysis of the 15 species belonging to the genus Debaryomyces. We found that the following species can be identified both quickly and correctly by direct sequence comparison of the ribosomal 5.8S-ITS region: D. carsonii, D. etchelsii, D. maramus, D. melissophilus, D. occidentalis and D. yamadae. In contrast, the species D. castellii, D. coudertii, D. hansenii, D. nepalensis, D. polymorphus, D. pseudopolymorphus, D. robertsiae, D. udenii and D. vanrijiae showed high sequence similarity in ribosomal regions with one or several species. In these cases, sequence comparison of the ACT1 gene is proposed to ensure unequivocal strain designation.
在本研究中，我们通过序列分析评估了德巴利酵母属的15个物种。我们发现，通过核糖体5.8S-ITS区域的直接序列比较，可以快速且准确地鉴定出以下物种：卡氏德巴利酵母、埃氏德巴利酵母、马拉德巴利酵母、嗜蜂德巴利酵母、西方德巴利酵母和山田德巴利酵母。相比之下，卡斯泰利德巴利酵母、库德特德巴利酵母、汉森德巴利酵母、尼泊尔德巴利酵母、多形德巴利酵母、假多形德巴利酵母、罗伯茨德巴利酵母、乌德尼德巴利酵母和范里吉德巴利酵母在核糖体区域与一个或多个物种表现出高度的序列相似性。在这些情况下，建议对ACT1基因进行序列比较，以确保菌株的明确鉴定。
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