Eady John J, Wortley Gary M, Wormstone Yvette M, Hughes Jackie C, Astley Siân B, Foxall Robert J, Doleman Joanne F, Elliott Ruan M
Institute of Food Research, Norwich Research Park, Colney, Norwich, United Kingdom.
Physiol Genomics. 2005 Aug 11;22(3):402-11. doi: 10.1152/physiolgenomics.00080.2005. Epub 2005 Jul 12.
The normal degree of intra- and interindividual variation in gene transcription profiles of healthy human tissues has not been extensively investigated. In the study described here, microarrays were employed to analyze gene transcription in peripheral blood mononuclear cells prepared from serial blood samples that had been obtained, at weekly intervals, from apparently healthy human volunteers. Transcript levels for the majority of genes examined were found to be remarkably consistent within samples from a single donor. Conversely, marked differences were observed in samples obtained from different donors. Genes that exhibited differential expression dependent on sex, age, body mass index, and the presence of varying proportions of different leukocyte subsets were identified. These results emphasize the important contributions of genetic and environmental factors, as well as varying representation of different cell types, in determining the overall gene transcriptional profiles of human tissues. However, the study also provides evidence that, within an individual, the gene transcription profiles of sampled tissues can be comparatively stable over time.
健康人体组织基因转录谱中个体内和个体间的正常变异程度尚未得到广泛研究。在本文所述的研究中，利用微阵列分析从明显健康的人类志愿者身上每周采集一次的系列血样制备的外周血单核细胞中的基因转录。在所检测的大多数基因中，发现来自单个供体的样本内转录水平非常一致。相反，在来自不同供体的样本中观察到明显差异。鉴定出了依赖于性别、年龄、体重指数以及不同白细胞亚群不同比例存在而表现出差异表达的基因。这些结果强调了遗传和环境因素以及不同细胞类型的不同占比在决定人体组织整体基因转录谱方面的重要作用。然而，该研究也提供了证据，即在个体内，采样组织的基因转录谱随时间可能相对稳定。
