Brett M M, McLauchlin J, Harris A, O'Brien S, Black N, Forsyth R J, Roberts D, Bolton F J
Health Protection Agency Food Safety Microbiology Laboratory, Centre for Infections, 61 Colindale Avenue, London NW9 5HT, UK 2Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK 3Health Protection Agency Communicable Disease Surveillance Centre, Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK 4Health Protection Agency North of Tyne Communicable Disease Control Unit, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE, UK 5Department of Child Health, University of Newcastle upon Tyne, Royal Victoria Infirmary, Newcastle upon Tyne NE1 4LP, UK.
J Med Microbiol. 2005 Aug;54(Pt 8):769-776. doi: 10.1099/jmm.0.46000-0.
Infant botulism was confirmed in a 5-month-old female by both isolation of Clostridium botulinum type B and by detection of type B botulinum neurotoxin in rectal washout and faeces. DNA fingerprinting of nine isolates from faeces yielded two different amplified-fragment length polymorphism (AFLP) patterns. C. botulinum was isolated from two of 14 food and drink items from the patient's home: C. botulinum type A was recovered from an opened container of dried rice pudding and C. botulinum type B from opened infant formula milk powder. Ten C. botulinum type B isolates from the opened infant formula yielded four AFLP patterns, two of which were indistinguishable from the clinical isolates. Fifteen unopened foods were tested and C. botulinum type B of a unique AFLP pattern was recovered from one unopened infant formula of the same batch as the opened container. It is suggested that multiple C. botulinum were present in both food and the intestine during infant botulism.
通过分离出B型肉毒杆菌以及在直肠冲洗液和粪便中检测到B型肉毒杆菌神经毒素，确诊一名5个月大的女婴患有婴儿肉毒中毒。对从粪便中分离出的9株菌株进行DNA指纹分析，得到了两种不同的扩增片段长度多态性（AFLP）图谱。从患者家中的14种食品和饮料中，有两种分离出了肉毒杆菌：从一个打开的干米布丁容器中分离出了A型肉毒杆菌，从打开的婴儿配方奶粉中分离出了B型肉毒杆菌。从打开的婴儿配方奶粉中分离出的10株B型肉毒杆菌产生了4种AFLP图谱，其中两种与临床分离株无法区分。对15种未开封食品进行了检测，从与打开容器同一批次的一份未开封婴儿配方奶粉中分离出了具有独特AFLP图谱的B型肉毒杆菌。提示在婴儿肉毒中毒期间，食品和肠道中均存在多种肉毒杆菌。
