Nakajima Kenji, Hirota Kikue, Nodasaka Yoshinobu, Yumoto Isao
Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Laboratory of Electron Microscopy, Graduate School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan.
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1525-1530. doi: 10.1099/ijs.0.63487-0.
Three indigo-reducing obligately alkaliphilic strains, M3(T), 41A and 41C, were isolated. The isolates grew at pH 9-12, but not at pH 7-8. They were Gram-positive, facultatively anaerobic, straight rod-shaped strains with peritrichous flagella. The isolates grew in 0-14% (w/v) NaCl, with optimum growth at 3-13%. They grew at temperatures between 10 and 45 degrees C, with optimum growth at around 30-37 degrees C. They did not hydrolyse starch or gelatin. DL-lactate was the major end-product from D-glucose. No quinones could be detected. The peptidoglycan type was A4beta, Orn-d-Asp. The major cellular fatty acids were C(16:0), C(16:1)7c and C(18:1)9c. The DNA G+C content was 42.6-43.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that the isolates belong to the genus Alkalibacterium. DNA-DNA hybridization revealed low similarity (less than 16%) of the isolates with respect to the two closest phylogenetically related strains, Alkalibacterium olivapovliticus and Alkalibacterium psychrotolerans. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic data and DNA-DNA relatedness, the isolates merit classification as a novel species of the genus Alkalibacterium, for which the name Alkalibacterium iburiense is proposed. The type strain is M3(T) (=JCM 12662(T)=NCIMB 14024(T)).
分离出了三株靛蓝还原专性嗜碱菌株，即M3(T)、41A和41C。这些分离株在pH 9至12的条件下生长，但在pH 7至8时不生长。它们为革兰氏阳性、兼性厌氧、直杆状菌株，具周生鞭毛。分离株能在0-14%（w/v）NaCl中生长，最适生长盐浓度为3-13%。它们在10至45℃之间生长，最适生长温度约为30-37℃。它们不水解淀粉或明胶。DL-乳酸是D-葡萄糖的主要终产物。未检测到醌类物质。肽聚糖类型为A4β，Orn-d-Asp。主要细胞脂肪酸为C(16:0)、C(16:1)7c和C(18:1)9c。DNA的G+C含量为42.6-43.2 mol%。基于16S rRNA基因序列数据的系统发育分析表明，这些分离株属于嗜碱杆菌属。DNA-DNA杂交显示，这些分离株与系统发育关系最密切的两株菌，即橄榄嗜碱杆菌和耐冷嗜碱杆菌的相似性较低（低于16%）。基于表型、化学分类特征、系统发育数据和DNA-DNA相关性分析结果，这些分离株应归为嗜碱杆菌属的一个新物种，为此提出名为伊布嗜碱杆菌的名称。模式菌株为M3(T)（=JCM 12662(T)=NCIMB 14024(T)）。
