Yumoto Isao, Hirota Kikue, Nodasaka Yoshinobu, Yokota Yuji, Hoshino Tamotsu, Nakajima Kenji
Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.
Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2379-2383. doi: 10.1099/ijs.0.63130-0.
A psychrotolerant, obligately alkaliphilic bacterium, IDR2-2(T), which is able to reduce indigo dye was isolated from a fermented polygonum indigo (Polygonum tinctorium Lour.) produced in Date, Hokkaido, using a traditional Japanese method. The isolate grew at pH 9-12 but not at pH 7-8. It was a Gram-positive, facultatively anaerobic, straight rod-shaped bacterium with peritrichous flagella. The isolate grew in 0-17 % (w/v) NaCl but not at NaCl concentrations higher than 18 % (w/v). Its major cellular fatty acids were C(14 : 0), C(16 : 0), C(16 : 1)9c and C(18 : 1)9c, and its DNA G+C content was 40.6 mol%. dl-lactic acid was the major end-product from d-glucose. No quinones could be detected. The peptidoglycan type was A4beta, Orn-d-Glu. A phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain IDR2-2(T) is a member of the genus Alkalibacterium. DNA-DNA hybridization revealed low relatedness (less than 25 %) between the isolate and two phylogenetically related strains, Alkalibacterium olivapovliticus and Marinilactibacillus psychrotolerans. On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA relatedness data, the isolate merits classification as a novel species, for which the name Alkalibacterium psychrotolerans sp. nov. is proposed. The type strain is IDR2-2(T) (=JCM 12281(T)=NCIMB 13981(T)).
一株耐冷嗜碱菌IDR2-2(T)，能够还原靛蓝染料，采用传统日本方法从北海道伊达市生产的发酵蓼蓝（蓼蓝）中分离得到。该菌株在pH 9 - 12条件下生长，在pH 7 - 8条件下不生长。它是革兰氏阳性、兼性厌氧、直杆状细菌，具周生鞭毛。该菌株在0 - 17%（w/v）NaCl中生长，但在NaCl浓度高于18%（w/v）时不生长。其主要细胞脂肪酸为C(14 : 0)、C(16 : 0)、C(16 : 1)9c和C(18 : 1)9c，DNA G+C含量为40.6 mol%。d-葡萄糖的主要终产物是dl-乳酸。未检测到醌类。肽聚糖类型为A4β，Orn-d-Glu。基于16S rRNA基因序列数据的系统发育分析表明，菌株IDR2-2(T)是嗜碱杆菌属的成员。DNA-DNA杂交显示该菌株与两个系统发育相关菌株嗜碱橄榄菌和耐冷海乳杆菌之间的相关性较低（低于25%）。基于表型特征、系统发育数据和DNA-DNA相关性数据，该菌株应分类为一个新物种，为此提出新名称嗜冷嗜碱杆菌（Alkalibacterium psychrotolerans sp. nov.）。模式菌株为IDR2-2(T)（=JCM 12281(T)=NCIMB 13981(T)）。
