Lau Stanley C K, Tsoi Mandy M Y, Li Xiancui, Plakhotnikova Ioulia, Dobretsov Sergey, Lau Ken W K, Wu Madeline, Wong Po-Keung, Pawlik Joseph R, Qian Pei-Yuan
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.
Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, People's Republic of China.
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1589-1592. doi: 10.1099/ijs.0.63661-0.
A Gram-negative, rod-shaped bacterium (designated strain UST030701-295(T)) with fast gliding motility was isolated from the surface of the sponge Lissodendoryx isodictyalis in the Bahamas. Colonies of UST030701-295(T) were yellow in colour, 2-4 mm in diameter, convex with a smooth surface and entire margins. UST030701-295(T) was heterotrophic, strictly aerobic and required NaCl for growth (1.0-4.0%). Growth was observed at pH 6.0-10.0 and at 12-44 degrees C. Phylogenetic analysis of the 16S rRNA gene sequence placed UST030701-295(T) within the genus Winogradskyella of the family Flavobacteriaceae, sharing 94.7-95.8% similarity with the three recognized members of the genus. The G+C content of the DNA was 32.8 mol% and the predominant fatty acids were iso-C(15:1), iso-C(15:0), iso-C(15:0) 2-OH, iso-C(15:0) 3-OH, iso-C(16:0) 3-OH, C(16:1)omega7 and iso-C(17:0) 3-OH (together representing 75.4% of the total); these data supported the affiliation of UST030701-295(T) to the genus Winogradskyella. UST030701-295(T) differed from the three recognized species of Winogradskyella in 7-17 traits. Molecular evidence together with phenotypic characteristics suggests that UST030701-295(T) represents a novel species within the genus Winogradskyella, for which the name Winogradskyella poriferorum sp. nov. is proposed. The type strain is UST030701-295(T) (=NRRL B-41101(T)=JCM 12885(T)).
从巴哈马群岛的等辐骨海绵（Lissodendoryx isodictyalis）表面分离出一种革兰氏阴性、杆状细菌（命名为UST030701-295(T)），具有快速滑动运动能力。UST030701-295(T)的菌落呈黄色，直径2-4毫米，凸起，表面光滑，边缘整齐。UST030701-295(T)是异养型、严格需氧型，生长需要NaCl（1.0-4.0%）。在pH 6.0-10.0和12-44℃条件下可观察到生长。对16S rRNA基因序列进行系统发育分析，结果表明UST030701-295(T)属于黄杆菌科（Flavobacteriaceae）的威诺格拉德斯基菌属（Winogradskyella），与该属三个已确认的成员相似度为94.7-95.8%。DNA的G+C含量为32.8 mol%，主要脂肪酸为异-C(15:1)、异-C(15:0)、异-C(15:0) 2-OH、异-C(15:0) 3-OH、异-C(16:0) 3-OH、C(16:1)ω7和异-C(17:0) 3-OH（共占总量的75.4%）；这些数据支持UST030701-295(T)隶属于威诺格拉德斯基菌属。UST030701-295(T)与威诺格拉德斯基菌属三个已确认的物种在7-17个特征上存在差异。分子证据和表型特征表明，UST030701-295(T)代表威诺格拉德斯基菌属内的一个新物种，为此提出新名称威诺格拉德斯基海绵菌（Winogradskyella poriferorum sp. nov.）。模式菌株为UST030701-295(T)（=NRRL B-41101(T)=JCM 12885(T)）。
