Yang Seung-Jo, Oh Hyun-Myung, Chung Sangyun, Cho Jang-Cheon
Division of Biology and Ocean Sciences, Inha University, Incheon, Republic of Korea.
J Microbiol. 2009 Oct;47(5):517-23. doi: 10.1007/s12275-009-0225-4. Epub 2009 Oct 24.
A marine bacterium, designated IMCC3175(T), was isolated from a seawater sample collected off the Antarctic coast. The strain was Gram-negative, obligately aerobic, carotenoid pigment-containing, and rod-shaped bacterium that divided by binary fission. As determined by 16S rRNA gene sequence comparisons, the most closely related genera were Formosa (92.9 approximately 93.3%), Bizionia (91.6 approximately 93.2%), Gaetbulibacter (91.5 approximately 92.8%), Sediminibacter (92.7%), Yeosuana (92.6%), Subsaximicrobium (92.1 approximately 92.2%), and Gillisia (89.5 approximately 92.2%). Phylogenese analysis based on 16S rRNA gene sequences showed that the strain formed a monophyletic clade together with the genera Sediminibacter and Subsaximicrobium but represented an independent phyletic line in this clade of the family Flavobacteriaceae. The DNA G+C content of the strain was 37.3 mol%. The major respiratory quinone was MK-6 and the predominant cellular fatty acids were C(16:1) omega7c and/or iso-C(15:0) 2-OH (12.8%), anteiso-C(15:0) (9.4%), and iso-C(16:1) (9.4%). Low 16S rRNA gene sequence similarity, formation of a distinct phylogenetic branch, and several phenotypic characteristics, including a narrow range of temperature and salinity for growth, differentiated strain IMCC3175(T) from other related genera in the family Flavobacteriaceae. Therefore the name Antarcticimonas flava gen. nov., sp. nov. is proposed, with strain IMCC3175(T) (=KCCM 42713(T) =NBRC 103398(T)) as the type strain.
从南极海岸采集的海水样本中分离出一株海洋细菌，命名为IMCC3175(T)。该菌株为革兰氏阴性菌，专性需氧，含类胡萝卜素色素，呈杆状，通过二分分裂进行繁殖。通过16S rRNA基因序列比较确定，与其关系最密切的属为福尔摩沙菌属（92.9%至93.3%）、比齐奥尼亚菌属（91.6%至93.2%）、加特布利杆菌属（91.5%至92.8%）、沉积杆菌属（92.7%）、丽水菌属（92.6%）、次萨克西微生物属（92.1%至92.2%）和吉利斯菌属（89.5%至92.2%）。基于16S rRNA基因序列的系统发育分析表明，该菌株与沉积杆菌属和次萨克西微生物属共同形成一个单系分支，但在黄杆菌科的这个分支中代表一个独立的进化谱系。该菌株的DNA G+C含量为37.3 mol%。主要呼吸醌为MK-6，主要细胞脂肪酸为C(16:1) ω7c和/或异-C(15:0) 2-OH（12.8%）、anteiso-C(15:0)（9.4%）和异-C(16:1)（9.4%）。16S rRNA基因序列相似性较低、形成独特的系统发育分支以及包括生长温度和盐度范围较窄在内的几个表型特征，使IMCC3175(T)菌株与黄杆菌科其他相关属区分开来。因此，提出新属名南极黄杆菌属（Antarcticimonas），新种名黄南极黄杆菌（Antarcticimonas flava），模式菌株为IMCC3175(T)（=KCCM 42713(T) =NBRC 103398(T)）。
