Lau Stanley C K, Tsoi Mandy M Y, Li Xiancui, Plakhotnikova Ioulia, Dobretsov Sergey, Wu Madeline, Wong Po-Keung, Pawlik Joseph R, Qian Pei-Yuan
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR.
Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR.
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):1059-1065. doi: 10.1099/ijs.0.64104-0.
Bacterial strains UST030701-097T and UST030701-084T were isolated from a marine sponge in the Bahamas. Both strains were pink-pigmented, Gram-negative, strictly aerobic and chemo-organotrophic. Cells of strain UST030701-097T were short, curved rods with fast-gliding motility, whereas those of strain UST030701-084T were straight rods with a less rapid gliding motion. The two strains had MK-7 as the major respiratory quinone and did not produce flexirubin-type pigments. The DNA G+C contents of strains UST030701-097T and UST030701-084T were 42.5 and 43.7 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains belonged to the family 'Flexibacteraceae' of the phylum Bacteroidetes. 16S rRNA gene sequence similarity between strains UST030701-097T and UST030701-084T was 95.0 %; their closest relative was [Marinicola] seohaensis, with 93.3 % and 96.0 % sequence similarity, respectively. Phylogenetic tree topology indicated that the two strains belonged to the same lineage, but were on separate branches. Whilst strain UST030701-084T and [Marinicola] seohaensis were found on one branch, strain UST030701-097T was in another branch that had no species with validly published names. Based on the polyphasic taxonomic data obtained in the present study, we propose that strain UST030701-097T represents a novel genus and that strain UST030701-084T represents a novel species in the phylum Bacteroidetes. The genus Fabibacter gen. nov. is proposed, with strain UST030701-097T (=NRRL B-41220T=JCM 13334T) as the type strain of the type species, Fabibacter halotolerans sp. nov. Strain UST030701-084T (=NRRL B-41219T=JCM 13337T) is proposed as the type strain of Roseivirga spongicola sp. nov. In an earlier study, it was suggested that the genus Marinicola is a later heterotypic synonym of the genus Roseivirga. However, a formal proposal to reclassify [Marinicola] seohaensis, the only member of the genus Marinicola, has not yet been made. The results of phylogenetic analyses in this study support the reclassification of [Marinicola] seohaensis as Roseivirga seohaensis comb. nov.
细菌菌株UST030701 - 097T和UST030701 - 084T是从巴哈马群岛的一种海洋海绵中分离出来的。这两种菌株均为粉红色素、革兰氏阴性、严格需氧且化能有机营养型。菌株UST030701 - 097T的细胞为短的弯曲杆菌，具有快速滑动运动能力，而菌株UST030701 - 084T的细胞为直杆菌，滑动运动速度较慢。这两种菌株以MK - 7作为主要呼吸醌，不产生柔韧菌素类色素。菌株UST030701 - 097T和UST030701 - 084T的DNA G + C含量分别为42.5和43.7 mol%。基于16S rRNA基因序列的系统发育分析表明，这两种菌株属于拟杆菌门“屈挠杆菌科”。菌株UST030701 - 097T和UST030701 - 084T之间的16S rRNA基因序列相似性为95.0%；它们最接近的亲缘种是[海栖菌属]西哈恩斯菌，序列相似性分别为93.3%和96.0%。系统发育树拓扑结构表明，这两种菌株属于同一谱系，但位于不同分支上。虽然菌株UST030701 - 084T和[海栖菌属]西哈恩斯菌位于一个分支上，但菌株UST030701 - 097T位于另一个没有有效发表名称物种的分支上。基于本研究获得的多相分类数据，我们提议菌株UST030701 - 097T代表一个新属，菌株UST030701 - 084T代表拟杆菌门中的一个新物种。提议建立新属法比杆菌属（Fabibacter gen. nov.），以菌株UST030701 - 097T（=NRRL B - 41220T = JCM 13334T）作为模式种嗜盐法比杆菌（Fabibacter halotolerans sp. nov.）的模式菌株。提议将菌株UST030701 - 084T（=NRRL B - 41219T = JCM 13337T）作为海绵玫瑰色菌（Roseivirga spongicola sp. nov.）的模式菌株。在早期研究中，有人提出海栖菌属是玫瑰色菌属的晚出异名。然而，尚未对海栖菌属的唯一成员[海栖菌属]西哈恩斯菌进行正式的重新分类提议。本研究的系统发育分析结果支持将[海栖菌属]西哈恩斯菌重新分类为西哈恩斯玫瑰色菌（Roseivirga seohaensis comb. nov.）。
