首个真正严格的互营丙酸氧化细菌，施氏泥杆菌新种（Pelotomaculum schinkii sp. nov.），与亨氏甲烷螺菌（Methanospirillum hungatei）共培养，以及泥杆菌属的修订描述。

The first true obligately syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov., co-cultured with Methanospirillum hungatei, and emended description of the genus Pelotomaculum.

作者信息

de Bok Frank A M, Harmsen Hermie J M, Plugge Caroline M, de Vries Maaike C, Akkermans Antoon D L, de Vos Willem M, Stams Alfons J M

机构信息

Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.

Department of Medical Microbiology, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.

出版信息

Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1697-1703. doi: 10.1099/ijs.0.02880-0.

DOI:10.1099/ijs.0.02880-0

PMID:16014504

Abstract

A Gram-positive, spore-forming, syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov. strain HH(T), was isolated as a co-culture with Methanospirillum hungatei JF-1(T) from anaerobic, freeze-dried granular sludge obtained from an upflow anaerobic sludge bed reactor treating sugar beet wastewater. The bacterium converted propionate to acetate in co-culture with Methanospirillum hungatei JF-1(T) or Methanobacterium formicicum MF(NT), but not in co-culture with Methanobrevibacter arboriphilus AZ. The organism could not be cultured axenically with any of the substrates tested and therefore can be considered as a (the first) true anaerobic syntrophic bacterium. The bacterium contained two distinct 16S rRNA gene sequences, with 96.8% sequence similarity, which were both expressed during syntrophic growth on propionate as revealed by fluorescent in situ hybridization. The most closely related organisms are Cryptanaerobacter phenolicus LR7.2(T), a bacterium that transforms phenol into benzoate, and Pelotomaculum thermopropionicum SI(T), a thermophilic, syntrophic propionate-oxidizing bacterium. Other related species belong to the Gram-positive, sulfate-reducing genus Desulfotomaculum. The type strain of Pelotomaculum schinkii is strain HH(T) (=ATCC BAA-615(T)=DSM 15200(T)).

摘要

从处理甜菜废水的上流式厌氧污泥床反应器获得的厌氧冻干颗粒污泥中，分离出一株革兰氏阳性、产芽孢、互营丙酸氧化细菌——新种施氏泥杆菌（Pelotomaculum schinkii）HH(T)菌株，它是与亨氏甲烷螺菌（Methanospirillum hungatei）JF-1(T)共培养得到的。该细菌与亨氏甲烷螺菌JF-1(T)或甲酸甲烷杆菌（Methanobacterium formicicum）MF(N)共培养时可将丙酸转化为乙酸，但与嗜树甲烷短杆菌（Methanobrevibacter arboriphilus）AZ共培养时则不能。该微生物无法在任何测试底物上进行纯培养，因此可被视为（首个）真正的厌氧互营细菌。通过荧光原位杂交发现，该细菌含有两个不同的16S rRNA基因序列，序列相似性为96.8%，在以丙酸为底物的互营生长过程中均有表达。与其亲缘关系最近的微生物是将苯酚转化为苯甲酸的酚隐厌氧杆菌（Cryptanaerobacter phenolicus）LR7.2(T)，以及嗜热互营丙酸氧化细菌嗜热丙酸泥杆菌（Pelotomaculum thermopropionicum）SI(T)。其他相关物种属于革兰氏阳性、硫酸盐还原的脱硫肠状菌属（Desulfotomaculum）。施氏泥杆菌的模式菌株是HH(T)菌株（=ATCC BAA-615(T)=DSM 15200(T)）。