Harmsen H J, Van Kuijk B L, Plugge C M, Akkermans A D, De Vos W M, Stams A J
Laboratory of Microbiology, Wageningen Agricultural University, The Netherlands.
Int J Syst Bacteriol. 1998 Oct;48 Pt 4:1383-7. doi: 10.1099/00207713-48-4-1383.
A syntrophic propionate-oxidizing bacterium, strain MPOBT, was isolated from a culture enriched from anaerobic granular sludge. It oxidized propionate syntrophically in co-culture with the hydrogen- and formate-utilizing Methanospirillum hungateii, and was able to oxidize propionate and other organic compounds in pure culture with sulfate or fumarate as the electron acceptor. Additionally, it fermented fumarate. 16S rRNA sequence analysis revealed a relationship with Syntrophobacter wolinii and Syntrophobacter pfennigii. The G + C content of its DNA was 60.6 mol%, which is in the same range as that of other Syntrophobacter species. DNA-DNA hybridization studies showed less than 26% hybridization among the different genomes of Syntrophobacter species and strain MPOBT. This justifies the assignment of strain MPOBT to the genus Syntrophobacter as a new species. The name Syntrophobacter fumaroxidans is proposed; strain MPOBT (= DSM 10017T) is the type strain.
从厌氧颗粒污泥富集培养物中分离出一株互营丙酸氧化细菌，菌株MPOBT。它在与利用氢气和甲酸的亨氏甲烷螺菌共培养时以互营方式氧化丙酸，并且在以硫酸盐或富马酸盐作为电子受体的纯培养物中能够氧化丙酸和其他有机化合物。此外，它还能发酵富马酸盐。16S rRNA序列分析表明其与沃氏互营杆菌和芬氏互营杆菌存在亲缘关系。其DNA的G + C含量为60.6 mol%，与其他互营杆菌属物种处于同一范围。DNA-DNA杂交研究表明，互营杆菌属不同基因组与菌株MPOBT之间的杂交率低于26%。这证明将菌株MPOBT归为互营杆菌属的一个新物种是合理的。提议将其命名为富马酸氧化互营杆菌；菌株MPOBT（= DSM 10017T）为模式菌株。
