Croquefer S, Renaudineau Y, Jousse S, Gueguen P, Ansart S, Saraux A, Youinou P
Laboratory of Immunology, Brest University Medical School Hospital, BP824, F29609 Brest, France.
Ann N Y Acad Sci. 2005 Jun;1050:170-5. doi: 10.1196/annals.1313.017.
In murine systemic lupus erythematosus (SLE) models, nephritogenic anti-dsDNA IgG has been shown to cross-react with a kidney antigen, alpha-actinin, and to be critical in renal pathogenesis. In humans, studies of anti-alpha-actinin antibodies (Abs) are scarce, and these antibodies remain to be evaluated. We have thus far tested sera from patients with SLE (n = 103), rheumatoid arthritis (RA, n = 93), and primary Sjögren syndrome (pSS, n = 34), and from healthy subjects (n = 160), for the presence of anti-alpha-actinin and anti-DNA Abs. The latter were tested using several methods [IIF on Crithidia luciliae (Crit) and ELISA using dsDNA]. Anti-alpha-actinin Abs were confirmed by Western blot. Sera from 23 of 103 SLE patients, 3 of 93 RA patients, 1 of 33 pSS patients, and 1 of 160 controls scored positive for anti-alpha-actinin Abs. In SLE, the positivity was significantly associated with anti-dsDNA reactivity (22 of 23): 19 of 23 sera were alpha-actinin-positive/dsDNA-positive and 13 were alpha-actinin-positive/Crit-positive. Few cases were alpha-actinin-positive/dsDNA-negative: 1 SLE, 3 RA, and 1 control. Furthermore, anti-alpha-actinin Abs have been detected at high level before or at the early stage of lupus nephritis when compared with active and inactive SLE without kidney manifestations.
在小鼠系统性红斑狼疮（SLE）模型中，已证明致肾炎性抗双链DNA IgG可与肾脏抗原α-辅肌动蛋白发生交叉反应，并且在肾脏发病机制中起关键作用。在人类中，抗α-辅肌动蛋白抗体（Abs）的研究很少，这些抗体仍有待评估。迄今为止，我们检测了SLE患者（n = 103）、类风湿性关节炎（RA，n = 93）、原发性干燥综合征（pSS，n = 34）以及健康受试者（n = 160）的血清中抗α-辅肌动蛋白和抗DNA Abs的存在情况。后者使用了几种方法进行检测[利什曼原虫（Crit）上的间接免疫荧光法（IIF）以及使用双链DNA的酶联免疫吸附测定法（ELISA）]。抗α-辅肌动蛋白Abs通过蛋白质印迹法进行确认。103例SLE患者中有23例、93例RA患者中有3例、33例pSS患者中有1例以及160例对照中有1例的血清抗α-辅肌动蛋白Abs检测呈阳性。在SLE中，阳性与抗双链DNA反应性显著相关（23例中的22例）：23例血清中有19例α-辅肌动蛋白阳性/双链DNA阳性，13例α-辅肌动蛋白阳性/Crit阳性。很少有病例是α-辅肌动蛋白阳性/双链DNA阴性：1例SLE、3例RA和1例对照。此外，与无肾脏表现的活动期和非活动期SLE相比，在狼疮性肾炎之前或早期就已检测到高水平的抗α-辅肌动蛋白Abs。
