Davtyan Tigran K, Manukyan Hayk M, Hakopyan Gagik S, Mkrtchyan Nana R, Avetisyan Samvel A, Galoyan Armen A
Department of Neurohormone Biochemistry, H. Buniatian Institute of Biochemistry, NAS RA, 5/1 Sevag Street, Yerevan 375014, Republic of Armenia.
Neurochem Res. 2005 Mar;30(3):297-309. doi: 10.1007/s11064-005-2603-6.
The AGAPEPAEPAQPGVY proline-rich polypeptide (PRP) was isolated from neurosecretory granules of the bovine neurohypophysis; it is produced by N. supraopticus and N. paraventricularis. PRP possesses immune-modulating activity, preventing the death of Gram-negative bacteria-infected mice. Here we show that PRP does not affect human peripheral blood neutrophlis and monocytes phagocytosis but dramatically enhances spontaneous or fMLP- and PMA-induced, and also phagocytosis-dependent, oxidative burst. We demonstrated the regulatory role of PRP on the oxidative burst induction of normal and relapsing inflammatory disease (Behcet's disease and familial Mediterranean fever) neutrophils and monocytes. Our results suggest a previously undescribed role for the hypothalamic peptide within primary activated neutrophils and monocytes, since we provide evidence that PRP can differentially regulate both chemotaxis- and phagocytosis-dependent oxidative burst in normal and inflammatory disease effector cells.
AGAPEPAEPAQPGVY富含脯氨酸的多肽（PRP）是从牛神经垂体的神经分泌颗粒中分离出来的；它由视上核和室旁核产生。PRP具有免疫调节活性，可防止革兰氏阴性菌感染的小鼠死亡。在这里我们表明，PRP不影响人外周血中性粒细胞和单核细胞的吞噬作用，但能显著增强自发的或fMLP和PMA诱导的以及吞噬作用依赖性的氧化爆发。我们证明了PRP对正常和复发性炎症性疾病（白塞病和家族性地中海热）中性粒细胞和单核细胞氧化爆发诱导的调节作用。我们的结果表明，下丘脑肽在原代活化的中性粒细胞和单核细胞中具有以前未描述的作用，因为我们提供的证据表明，PRP可以差异性地调节正常和炎症性疾病效应细胞中趋化作用和吞噬作用依赖性的氧化爆发。
