Bonfield T L, Colton E, Anderson J M
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106-4907.
J Biomed Mater Res. 1992 Apr;26(4):457-65. doi: 10.1002/jbm.820260404.
The studies presented in this manuscript were based upon the hypothesis that monocytes/macrophages selectively produce cytokines and growth factors due to their interactions with polymers and proteins which are adsorbed to their surfaces. These factors in turn selectively influence the ability of fibroblasts to proliferate. The factors which influence fibroblast proliferation were released from monocytes incubated with polymers: Biomer, polydimethylsiloxane (PDMS), polyethylene (PE), expanded polytetrafluoroethylene (ePTFE), Dacron, and control polystyrene with and without preadsorption with physiological concentrations of IgG, fibrinogen, fibronectin, hemoglobin, or albumin. No simple correlation was found between adsorbed protein, biomedical polymer, and the ability of monocytes to produce growth factors and cytokines which influence fibroblast proliferation. This is evidence for selective protein-polymer interactions which in turn selectively activate monocytes to produce variable cell cycle competence and progression factors controlling fibroblast growth.
单核细胞/巨噬细胞由于与吸附在其表面的聚合物和蛋白质相互作用，会选择性地产生细胞因子和生长因子。这些因子进而选择性地影响成纤维细胞的增殖能力。影响成纤维细胞增殖的因子是从与聚合物一起孵育的单核细胞中释放出来的：生物材料、聚二甲基硅氧烷（PDMS）、聚乙烯（PE）、膨体聚四氟乙烯（ePTFE）、涤纶，以及有或没有用生理浓度的免疫球蛋白G、纤维蛋白原、纤连蛋白、血红蛋白或白蛋白预吸附的对照聚苯乙烯。在吸附的蛋白质、生物医学聚合物以及单核细胞产生影响成纤维细胞增殖的生长因子和细胞因子的能力之间，未发现简单的相关性。这证明了选择性的蛋白质 - 聚合物相互作用，进而选择性地激活单核细胞产生控制成纤维细胞生长的可变细胞周期能力和进展因子。
