Bonfield T L, Colton E, Marchant R E, Anderson J M
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
J Biomed Mater Res. 1992 Jul;26(7):837-50. doi: 10.1002/jbm.820260702.
These studies evaluate the effect of biomedical polymers: Biomer, polydimethyl-siloxane (PDMS), polyethylene, expanded polytetrafluoroethylene (ePTFE), Dacron, and the control polystyrene with or without adsorbed proteins IgG, fibrinogen, and fibronectin on the ability of activated human monocytes/macrophages to produce Interleukin 1 Beta (IL-1-B), Interleukin 6 (IL-6), and Tumor Necrosis Factor Alpha (TNF-A). Monocytes/macrophages incubated on biomedical polymers with or without protein preadsorption produce variable levels of IL-1-B, IL-6, and TNF-A dependent on the polymer and adsorbed protein. IL-6 was produced in the greatest quantity and was the most influenced by protein adsorption. ePTFE and PDMS polymers were least stimulating while polystyrene was the most stimulating of monocyte activity. Adsorbed IgG consistently altered the ability of the polymers to activate monocytes/macrophages to produce cytokines. These studies provide important insight into conditions which modulate monocyte/macrophage activity in response to protein preadsorbed biomedical polymers.
这些研究评估了生物医学聚合物（Biomer、聚二甲基硅氧烷（PDMS）、聚乙烯、膨体聚四氟乙烯（ePTFE）、涤纶）以及对照聚苯乙烯在有或没有吸附蛋白质免疫球蛋白G（IgG）、纤维蛋白原和纤连蛋白的情况下，对活化的人单核细胞/巨噬细胞产生白细胞介素1β（IL-1-β）、白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）能力的影响。在有或没有蛋白质预吸附的生物医学聚合物上孵育的单核细胞/巨噬细胞产生的IL-1-β、IL-6和TNF-α水平各不相同，这取决于聚合物和吸附的蛋白质。IL-6的产生量最大，且受蛋白质吸附的影响最大。ePTFE和PDMS聚合物对单核细胞活性的刺激最小，而聚苯乙烯的刺激最大。吸附的IgG始终会改变聚合物激活单核细胞/巨噬细胞产生细胞因子的能力。这些研究为调节单核细胞/巨噬细胞对蛋白质预吸附生物医学聚合物作出反应的活性的条件提供了重要见解。
