Tognana Enrico, Padera Robert F, Chen Fen, Vunjak-Novakovic Gordana, Freed Lisa E
Division of Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Osteoarthritis Cartilage. 2005 Oct;13(10):896-905. doi: 10.1016/j.joca.2005.05.003. Epub 2005 Jul 12.
Development and remodeling of engineered cartilage-explant composites were studied in vitro and in vivo.
Individual and interactive effects of cell chondrogenic potential (primary or fifth passage bovine calf chondrocytes), scaffold degradation rate (hyaluronan benzyl ester or polyglycolic acid), and adjacent tissue cell activity and architecture (vital trabecular bone (VB), articular cartilage (AC), devitalized bone (DB) or digested cartilage (DC)) were evaluated over 8 weeks in vitro (bioreactor cultures) and in vivo (ectopic implants).
In vitro, significant effects of cell type on construct adhesive strength (P<0.001) and scaffold type on adhesive strength (P<0.001), modulus (P=0.014), glycosaminoglycans (GAG) (P<0.001), and collagen (P=0.039) were observed. Chondrogenesis was best when the scaffold degradation rate matched the extracellular matrix deposition rate. In vivo, adjacent tissue type affected adhesive strength (P<0.001), modulus (P<0.001), and GAG (P<0.001) such that 8-week values obtained for bone (VB and DB) were higher than for cartilage (AC). In the AC/construct group, chondrogenesis appeared attenuated in the region of the construct close to the AC. In contrast, in the VB/construct group, a 500 microm thick zone of mature hyaline-like cartilage formed at the interface, and signs of active remodeling were present in the bone that included osteoclastic and osteoblastic activity and trabecular rebuttressing; these features were not present in the DB group or in vitro.
Development and remodeling of composites based on engineered cartilage were mediated in vitro by cell chondrogenic potential and scaffold degradation rate, and in vivo by type of adjacent tissue and time.
在体外和体内研究工程化软骨-植入物复合材料的发育和重塑。
评估细胞软骨生成潜能（原代或第5代牛犊软骨细胞）、支架降解率（透明质酸苄酯或聚乙醇酸）以及相邻组织细胞活性和结构（活骨小梁（VB）、关节软骨（AC）、失活骨（DB）或消化软骨（DC））的个体及相互作用效应，研究为期8周，包括体外（生物反应器培养）和体内（异位植入）研究。
在体外，观察到细胞类型对构建体黏附强度有显著影响（P<0.001），支架类型对黏附强度（P<0.001）、模量（P=0.014）、糖胺聚糖（GAG）（P<0.001）和胶原蛋白（P=0.039）有显著影响。当支架降解率与细胞外基质沉积率匹配时，软骨生成效果最佳。在体内，相邻组织类型影响黏附强度（P<0.001）、模量（P<0.001）和GAG（P<0.001），使得骨（VB和DB）在8周时的值高于软骨（AC）。在AC/构建体组中，构建体靠近AC区域的软骨生成似乎减弱。相反，在VB/构建体组中，在界面处形成了一个500微米厚的成熟透明样软骨区，并且在骨中出现了活跃重塑的迹象，包括破骨细胞和成骨细胞活性以及骨小梁强化；这些特征在DB组或体外均未出现。
基于工程化软骨的复合材料的发育和重塑在体外由细胞软骨生成潜能和支架降解率介导，在体内由相邻组织类型和时间介导。
