Rankin Susan M, Kwok Rodney, Seymour Michelle L, Shaon Rahman U, Tobe Stephen S
Department of Biology, Allegheny College, Meadville, PA 16335, USA.
Comp Biochem Physiol B Biochem Mol Biol. 2005 Sep;142(1):113-22. doi: 10.1016/j.cbpc.2005.06.006.
Manduca sexta allatotropin (Manse-AT) was first isolated on the basis of its ability to stimulate production of juvenile hormone in that insect. We examined whether this neuropeptide affects corpus allatum activity and visceral muscle contraction in adult females of the earwig, Euborellia annulipes. We also assessed the presence of allatotropin-like material in tissues using immunocytochemistry. Manse-AT at 1 nM to 10 muM stimulated juvenile hormone production in vitro by glands of low activity from 2-day virgin females. In glands of high activity from 12-day mated females, 1 and 100 nM allatotropin were effective, but 10 muM was not. Similarly, hindguts of 2-day and 12-day females significantly increased in motility in vitro in response to Manse-AT. A monoclonal antibody to Manse-AT was used to demonstrate allatotropin-like material throughout the nervous system of 2-day, virgin females. Immunoreactivity was most pronounced within varicosities of the corpora cardiaca and perisympathetic organs. No immunofluorescence was observed in gut tissue. Lastly, we showed that extract of retrocerebral complexes also enhanced in vitro hindgut motility from 2-day virgin females, in a dose-dependent manner. These results indicate material similar to M. sexta allatotropin in female earwigs and that such peptides may modulate juvenile hormone biosynthesis and visceral muscle contractions. Sensitivity to the peptides may change with physiological stage.
烟草天蛾促咽侧体素（Manse - AT）最初是根据其刺激该昆虫体内保幼激素产生的能力而分离出来的。我们研究了这种神经肽是否会影响环纹大蠼螋成年雌性个体的咽侧体活性和内脏肌肉收缩。我们还使用免疫细胞化学方法评估了组织中促咽侧体素样物质的存在情况。1 nM至10 μM的Manse - AT可刺激2日龄未交配雌性低活性腺体在体外产生保幼激素。对于12日龄已交配雌性的高活性腺体，1 nM和100 nM的促咽侧体素有效，但10 μM无效。同样，2日龄和12日龄雌性的后肠在体外对Manse - AT的反应中，运动性显著增强。一种针对Manse - AT的单克隆抗体被用于证明2日龄未交配雌性整个神经系统中存在促咽侧体素样物质。免疫反应性在心脏体和交感神经周围器官的膨大部最为明显。在肠道组织中未观察到免疫荧光。最后，我们表明脑复合体提取物也能以剂量依赖的方式增强2日龄未交配雌性的体外后肠运动性。这些结果表明雌性蠼螋体内存在与烟草天蛾促咽侧体素类似的物质，并且此类肽可能调节保幼激素的生物合成和内脏肌肉收缩。对这些肽的敏感性可能会随着生理阶段而变化。
