Oliveira Vinícius A, Abreu Lauriane G, Ferriani Rui A, Reis Rosana M, Moura Marcos D
Sector of Human Reproduction, Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av. Dos Bandeirantes 3900, 14049-900 Ribeirão Preto, São Paulo, Brazil.
Gynecol Endocrinol. 2005 May;20(5):284-8. doi: 10.1080/09513590500097952.
Vascular endothelial growth factor (VEGF), a potent angiogenic factor that is altered in endometriosis, supports the immunological mechanism involved in this disease. The aim of the present study was to assess VEGF concentration in the plasma, follicular fluid (FF) and culture medium (CM) of granulosa cells from patients with endometriosis submitted to in vitro fertilization (IVF). A case-control study was conducted on 14 patients with endometriosis and 14 women without endometriosis submitted to IVF. Peripheral blood samples were collected before and after administration of human chorionic gonadotropin (hCG), in addition to FF and CM samples. Plasma VEGF levels increased after hCG administration in women with endometriosis and in controls, but were significant only in controls. VEGF levels were lower in FF but were significantly increased in the CM of patients with endometriosis. There was no correlation between VEGF and age, response to ovarian stimulation, oocyte or embryo quality, and pregnancy result. The increase of VEGF levels after hCG in both groups demonstrated a positive effect of this hormone on VEGF. VEGF in the FF and CM presented opposite results in endometriosis, suggesting that granulosa cells may show a different behavior in vivo and in vitro.
血管内皮生长因子（VEGF）是一种在子宫内膜异位症中发生改变的强效血管生成因子，它支持该疾病所涉及的免疫机制。本研究的目的是评估接受体外受精（IVF）的子宫内膜异位症患者的血浆、卵泡液（FF）和颗粒细胞培养基（CM）中的VEGF浓度。对14例接受IVF的子宫内膜异位症患者和14例无子宫内膜异位症的女性进行了病例对照研究。除了FF和CM样本外，在注射人绒毛膜促性腺激素（hCG）前后采集外周血样本。子宫内膜异位症患者和对照组女性在注射hCG后血浆VEGF水平均升高，但仅在对照组中具有统计学意义。FF中的VEGF水平较低，但子宫内膜异位症患者的CM中VEGF水平显著升高。VEGF与年龄、卵巢刺激反应、卵母细胞或胚胎质量以及妊娠结果之间没有相关性。两组在注射hCG后VEGF水平的升高表明该激素对VEGF有积极作用。FF和CM中的VEGF在子宫内膜异位症中呈现相反的结果，表明颗粒细胞在体内和体外可能表现出不同的行为。
