Alaseirlis Dimosthenis A, Li Yunxia, Cilli Feridun, Fu Freddie H, Wang James H-C
Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA.
Connect Tissue Res. 2005;46(1):12-7. doi: 10.1080/03008200590935501.
Tissue inflammation is essential in the healing process, but its effect on the quality of the healing tissue is not clear. This study determines the effect of decreasing early inflammation during wound healing in genetic deficient mice on collagen fibril diameter. Two strains of mice were used: three C3H/HeJ mice and three C3H/HeN mice for each of two time points (7 and 14 days postinjury). C3H/HeJ mice have a genetic deficiency in the production of tumor necrosis factor by macrophages and other cytokines in response to endotoxin, and C3H/HeN mice have no genetic deficiency. The right patellar tendon of both mouse strains was transversely transected, whereas the left patellar tendon was left intact for control. After 7 and 14 days, both right and left patellar tendons were harvested, and tendon samples were examined with transmission electron microscopy. We found that at 7 days, transected tendons of C3H/HeJ mice exhibited on average 1.6 times larger collagen fibril diameters than transected C3H/HeN tendons, whereas at 14 days, collagen fibril diameters of the C3H/HeJ mice were 1.3 times that of C3H/HeN mice. Also, at both 7 days and 14 days, collagen fibrils in C3H/HeJ mice appeared more organized than C3H/HeN mice. In addition, control tendons in both mouse strains showed no significant differences in collagen fibril diameter and organization. Therefore, these results suggest that decreasing the inflammatory response in the early stages of tendon wound healing enhances the quality of the healing tendon through increased collagen fiber diameter and better organization.
组织炎症在愈合过程中至关重要，但其对愈合组织质量的影响尚不清楚。本研究确定了在基因缺陷小鼠伤口愈合过程中减少早期炎症对胶原纤维直径的影响。使用了两种品系的小鼠：在两个时间点（受伤后7天和14天），每种品系各有三只C3H/HeJ小鼠和三只C3H/HeN小鼠。C3H/HeJ小鼠在巨噬细胞产生肿瘤坏死因子及其他细胞因子对内毒素作出反应方面存在基因缺陷，而C3H/HeN小鼠没有基因缺陷。两种品系小鼠的右髌腱均被横向切断，而左髌腱保持完整作为对照。7天和14天后，采集左右髌腱，并用透射电子显微镜检查肌腱样本。我们发现，在7天时，C3H/HeJ小鼠横断肌腱的胶原纤维直径平均比C3H/HeN小鼠横断肌腱大1.6倍，而在14天时，C3H/HeJ小鼠的胶原纤维直径是C3H/HeN小鼠的1.3倍。此外，在7天和14天时，C3H/HeJ小鼠的胶原纤维比C3H/HeN小鼠的更有序。另外，两种品系小鼠的对照肌腱在胶原纤维直径和排列上均无显著差异。因此，这些结果表明，在肌腱伤口愈合早期降低炎症反应可通过增加胶原纤维直径和更好的排列来提高愈合肌腱的质量。
