Kilian Olaf, Alt Volker, Heiss Christian, Jonuleit Tarja, Dingeldein Elvira, Flesch Ingo, Fidorra Ursula, Wenisch Sabine, Schnettler Reinhard
Department of Trauma Surgery, University of Giessen, Giessen, Germany.
Growth Factors. 2005 Jun;23(2):125-33. doi: 10.1080/08977190500126306.
Vascular endothelial growth factor (VEGF) plays a key role for the interaction of osteoblasts and endothelial cells and, therefore, is an important factor for the osteointegration of bone substitutes. The aim of the current work was to study the effects of platelet growth factors (PLF) on new blood vessel formation and VEGF-receptors expression pattern in bone defects filled with nanocrystalline hydroxyapatite (HA) paste in miniature-pigs. Conventional histology, RT-PCR for VEGF and receptors mRNA, Western blot analysis, immunohistochemical staining and quantitative assessment of newly formed vessels was performed. HA enriched with platelet growth factor (HA/PLF+) led to an up-regulation of VEGF-R1 synthesis, a slightly enhanced number of newly formed vessels with higher sprouting activity compared with HA without PLF (HA/PLF-) filling defects. These observation are most likely attributable to a stimulating effect of TGF-ss from the platelet factor on VEGF expression in osteoblasts.
血管内皮生长因子（VEGF）在成骨细胞与内皮细胞的相互作用中起关键作用，因此是骨替代物骨整合的重要因素。本研究的目的是研究血小板生长因子（PLF）对小型猪纳米晶羟基磷灰石（HA）糊剂填充骨缺损中新生血管形成和VEGF受体表达模式的影响。进行了常规组织学、VEGF及其受体mRNA的RT-PCR、蛋白质免疫印迹分析、免疫组织化学染色以及新生血管的定量评估。与未填充PLF的HA（HA/PLF-）相比，富含血小板生长因子的HA（HA/PLF+）导致VEGF-R1合成上调，新生血管数量略有增加，且具有更高的发芽活性。这些观察结果很可能归因于血小板因子中的TGF-β对成骨细胞中VEGF表达的刺激作用。
