Macek J, Ptácek P
Pharmakl s.r.o., Prague, Czech Republic.
J Chromatogr B Biomed Appl. 1995 Nov 17;673(2):316-9. doi: 10.1016/0378-4347(95)00263-5.
A new method for the determination of ofloxacin in human plasma was developed. Plasma proteins were precipitated with acetonitrile, the supernatant concentrated and injected into a reversed-phase C18 column. Enoxacin was used as an internal standard. The fluorimetric detection was performed at 282 nm for excitation and 450 nm for emission. Limit of quantitation was 20 ng/ml and the calibration curve was linear up to 6900 ng/ml.
建立了一种测定人血浆中氧氟沙星的新方法。用乙腈沉淀血浆蛋白，将上清液浓缩后注入反相C18柱。以依诺沙星为内标。荧光检测在激发波长282nm和发射波长450nm下进行。定量限为20ng/ml，校准曲线在6900ng/ml范围内呈线性。
