Manti L, Durante M, Cirrone G A P, Grossi G, Lattuada M, Pugliese M, Sabini M G, Scampoli P, Valastro L, Gialanella G
Department of Physical Sciences, University of Naples Federico II, and National Institute for Nuclear Physics (INFN), Section of Naples, Italy.
Int J Radiat Biol. 2005 Feb;81(2):147-55. doi: 10.1080/09553000500091188.
The aim was to evaluate the effect of modelled microgravity on radiation-induced chromosome aberrations (CAs). G0 peripheral blood lymphocytes were exposed to 60 MeV protons or 250 kVp X-rays in the dose range 0-6 Gy, and allowed to repair DNA damage for 24 h under either normal gravity or microgravity modelled by the NASA-designed rotating-wall bioreactor. Cells were then stimulated to proliferate by phytohaemagglutinin (PHA) under normal gravity conditions and prematurely condensed chromosomes were harvested after 48 h. CAs were scored in chromosomes 1 and 2 by fluorescence in-situ hybridization. Proliferation gravisensitivity was examined by cell growth curves and by morphological evaluation of mitogen-induced activation. Cell replication rounds were monitored by bromodeoxyuridine labelling. Modelled microgravity markedly reduced PHA-mediated lymphocyte blastogenesis and cell growth. However, no significant differences between normal gravity and modelled microgravity were found in the dose-response curves for the induction of aberrant cells or total interchromosomal exchange frequency. Rotating-wall bioreactor-based microgravity reproduced space-related alterations of mitogen stimulation in human lymphocytes but did not affect the yield of CAs induced by low-linear energy transfer radiation.
目的是评估模拟微重力对辐射诱导的染色体畸变（CA）的影响。将G0期外周血淋巴细胞暴露于剂量范围为0 - 6 Gy的60 MeV质子或250 kVp X射线下，并使其在正常重力或由美国国家航空航天局设计的旋转壁生物反应器模拟的微重力条件下修复DNA损伤24小时。然后在正常重力条件下用植物血凝素（PHA）刺激细胞增殖，48小时后收获早熟凝集染色体。通过荧光原位杂交对1号和2号染色体中的CA进行评分。通过细胞生长曲线和对有丝分裂原诱导激活的形态学评估来检测增殖严重敏感性。通过溴脱氧尿苷标记监测细胞复制轮次。模拟微重力显著降低了PHA介导的淋巴细胞增殖和细胞生长。然而，在异常细胞诱导或总染色体间交换频率的剂量反应曲线中，未发现正常重力和模拟微重力之间存在显著差异。基于旋转壁生物反应器的微重力再现了人类淋巴细胞中有丝分裂原刺激的与空间相关的变化，但不影响低线性能量传递辐射诱导的CA的产量。
