Juhel-Gaugain Murielle, Fourmond Marie-Pierre, Delepine Bernard, Laurentie Michel, Brigitte Roudaut, Sanders Pascal
French Food Safety Agency, Community Referance Laboratory for Antimicrobial Residues in Food from Animal Origin, Fougères, France.
Food Addit Contam. 2005 Mar;22(3):221-3. doi: 10.1080/02652030500058494.
Two interlaboratory studies were organized in 2002-2003 in order to check the proficiency of laboratories in confirming the presence of sulfonamide residues in muscle and milk. These studies involved 25 EU National Reference Laboratories (NRLs) from 21 different European Countries in charge of statutory monitoring of antimicrobial residues in food of animal origin at a national level. The study was conducted according to international and national guidelines by the Community Reference Laboratory (CRL) in charge of antimicrobial substances. Four different test matrices of sheep muscle and four different test matrices of bovine milk containing different sulfonamide substances were prepared and sent to the participants. Each participant was asked to use his own routine confirmatory method and to analyse each sample in triplicate within a period of about six weeks during which the stability of the materials was checked by the organizer. The sulfonamide content of each material was determined by calculating the robust means of all the results and the deviation of the results from the assigned values was assessed by calculating Z-scores. Overall, results were satisfactory, particularly considering that it was the first proficiency test dealing with sulfonamides organised by the Community Reference Laboratory.
2002年至2003年组织了两项实验室间研究，以检验各实验室确认肌肉和牛奶中磺胺类药物残留的能力。这些研究涉及来自21个不同欧洲国家的25个欧盟国家参考实验室（NRL），这些实验室负责在国家层面法定监测动物源性食品中的抗菌药物残留。该研究由负责抗菌物质的欧盟参考实验室（CRL）按照国际和国家指南进行。制备了含有不同磺胺类物质的四种不同绵羊肌肉测试基质和四种不同牛乳测试基质，并分发给参与者。要求每位参与者使用自己的常规确证方法，在约六周的时间内对每个样品进行三次分析，在此期间组织者会检查材料的稳定性。通过计算所有结果的稳健均值来确定每种材料的磺胺类药物含量，并通过计算Z分数评估结果与指定值的偏差。总体而言，结果令人满意，特别是考虑到这是欧盟参考实验室组织的首次磺胺类药物能力验证试验。
