Gulle H, Schoel B, Chiplunkar S, Gangal S, Deo M G, Kaufmann S H
Department of Immunology, University of Ulm, Germany.
Int J Lepr Other Mycobact Dis. 1992 Mar;60(1):44-53.
Sonicated extracts of Mycobacterium leprae were separated by two-dimensional gel electrophoresis and electroeluted into 400 distinct soluble fractions. These fractions were probed with T lymphocytes from leprosy patients of different disease types, healthy contacts, and unexposed healthy individuals. Proliferative responses were visualized using three-dimensional stimulation profiles. T cells from many patients and contacts responded to a multitude of antigen fractions of different molecular masses and isoelectric points. T cells from unexposed individuals gave significant responses to lysates or whole organisms of M. leprae, but no or only marginal responses to separated antigen fractions. T cells of polar tuberculoid (TT) and the majority of polar lepromatous (LL) leprosy patients responded only to separated antigen fractions but not to lysates or whole organisms of M. leprae. The remaining LL patients were totally unresponsive and even failed to respond to separated M. leprae fractions. Thus, in some leprosy patients unresponsiveness to M. leprae seems to be caused by distinct components and can be broken by using separated antigen fractions; whereas in others, anergy remains. T cells of borderline tuberculoid (BT) patients, who were under chemotherapy, responded to separated antigen fractions as well as to lysates of M. leprae organisms. In contrast, BT patients who were untreated failed to react with any of the M. leprae preparations. Similarly, T cells of the majority of LL patients responding to separated fractions were under chemotherapy; whereas T cells from untreated LL patients gave no or only marginal responses to any of the M. leprae antigen preparations. These findings suggest some linkage between the degree of T-cell responsiveness and antileprosy drug treatment.
将麻风分枝杆菌的超声提取物通过二维凝胶电泳进行分离，并电洗脱到400个不同的可溶性组分中。用来自不同疾病类型的麻风病人、健康接触者和未接触过麻风杆菌的健康个体的T淋巴细胞对这些组分进行检测。使用三维刺激图谱观察增殖反应。许多患者和接触者的T细胞对多种不同分子量和等电点的抗原组分有反应。未接触过麻风杆菌的个体的T细胞对麻风分枝杆菌的裂解物或完整菌体有显著反应，但对分离的抗原组分无反应或仅有微弱反应。极型结核样型（TT）麻风患者和大多数极型瘤型（LL）麻风患者的T细胞仅对分离的抗原组分有反应，而对麻风分枝杆菌的裂解物或完整菌体无反应。其余的LL患者完全无反应，甚至对分离的麻风分枝杆菌组分也无反应。因此，在一些麻风患者中，对麻风分枝杆菌无反应似乎是由特定成分引起的，使用分离的抗原组分可打破这种无反应状态；而在另一些患者中，无反应状态仍然存在。正在接受化疗的界线类结核样型（BT）麻风患者的T细胞对分离的抗原组分以及麻风分枝杆菌菌体的裂解物有反应。相比之下，未接受治疗的BT患者对任何麻风分枝杆菌制剂均无反应。同样，大多数对分离组分有反应的LL患者的T细胞正在接受化疗；而未接受治疗的LL患者的T细胞对任何麻风分枝杆菌抗原制剂均无反应或仅有微弱反应。这些发现提示T细胞反应程度与抗麻风药物治疗之间存在某种联系。
