Oyane Ayako, Uchida Masaki, Onuma Kazuo, Ito Atsuo
Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Biomaterials. 2006 Jan;27(2):167-75. doi: 10.1016/j.biomaterials.2005.06.001.
We have previously reported that a laminin-apatite composite layer is formed on an ethylene-vinyl alcohol copolymer (EVOH) in a laminin-containing calcium phosphate (LCP) solution. In this work, the stability of the LCP solution and growth process of the laminin-apatite composite layer have been investigated. Dynamic light scattering technique revealed that the LCP solution was stable for periods as long as 24 h; it did not induce homogeneous precipitation of laminin or calcium phosphates in the solution. Analysis of the EVOH surface and the LCP solution showed that the laminin-apatite composite layer was formed via coprecipitation of laminin and apatite on the EVOH plate, i.e., spontaneous growing of apatite and simultaneous immobilization of laminin molecules or laminin-calcium phosphate nano-complexes onto its surface. Transmission electron microscopy also revealed that the laminin molecules in the resulting composite layer were not localized or aggregated, but were dispersed on a nano-scale in the entire layer. Because of this nano-composite structure, a large number of laminin molecules were stably immobilized on the EVOH plate. This may be responsible for the excellent cell adhesion properties of this type of composite material.
我们之前报道过，在含层粘连蛋白的磷酸钙（LCP）溶液中，乙烯 - 乙烯醇共聚物（EVOH）上会形成层粘连蛋白 - 磷灰石复合层。在这项工作中，研究了LCP溶液的稳定性以及层粘连蛋白 - 磷灰石复合层的生长过程。动态光散射技术表明，LCP溶液在长达24小时的时间内是稳定的；它不会在溶液中诱导层粘连蛋白或磷酸钙的均匀沉淀。对EVOH表面和LCP溶液的分析表明，层粘连蛋白 - 磷灰石复合层是通过层粘连蛋白和磷灰石在EVOH板上的共沉淀形成的，即磷灰石的自发生长以及层粘连蛋白分子或层粘连蛋白 - 磷酸钙纳米复合物同时固定在其表面。透射电子显微镜还显示，所得复合层中的层粘连蛋白分子没有局部化或聚集，而是在整个层中以纳米尺度分散。由于这种纳米复合结构，大量层粘连蛋白分子稳定地固定在EVOH板上。这可能是这种复合材料具有优异细胞粘附特性的原因。
