Human Technology Research Institute, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
J R Soc Interface. 2013 Feb 13;10(81):20130014. doi: 10.1098/rsif.2013.0014. Print 2013 Apr 6.
Apatite (Ap), laminin-apatite composite (L5Ap, L10Ap, L20Ap and L40Ap) and albumin-apatite (AlbAp) composite layers were prepared on titanium (Ti) using a supersaturated calcium phosphate solution supplemented with laminin (0, 5, 10, 20 and 40 μg ml(-1)) or albumin (800 μg ml(-1)). With an increase in the concentrations of laminin in the supersaturated calcium phosphate solutions, the amounts of laminin immobilized on the Ti increased. The number of human umbilical vein endothelial cells (HUVECs) adhered to the laminin-apatite composite layers were remarkably higher than those to the untreated Ti, Ap layer and AlbAp composite layer. The number of cells adhered to the L40Ap was 4.3 times the untreated Ti. Moreover, cells adhered to the laminin-apatite composite layers showed significantly higher cell retention under the physiological shear stress for 1 h and 2 h than those to the untreated Ti, Ap layer and AlbAp composite layer. The number of cells remaining on the L40Ap under the physiological shear stress for 2 h was 9.5 times that of the untreated Ti. The laminin-apatite composite layer is a promising interfacial layer for endothelialization of blood-contacting materials.
磷灰石（Ap）、层粘连蛋白-磷灰石复合层（L5Ap、L10Ap、L20Ap 和 L40Ap）和白蛋白-磷灰石（AlbAp）复合层是在钛（Ti）上通过补充有层粘连蛋白（0、5、10、20 和 40μg/ml）或白蛋白（800μg/ml）的过饱和磷酸钙溶液制备的。随着过饱和磷酸钙溶液中层粘连蛋白浓度的增加，固定在 Ti 上的层粘连蛋白的量增加。黏附在层粘连蛋白-磷灰石复合层上的人脐静脉内皮细胞（HUVECs）数量明显高于未经处理的 Ti、Ap 层和 AlbAp 复合层。黏附在 L40Ap 上的细胞数量是未经处理的 Ti 的 4.3 倍。此外，在生理剪切力下 1 小时和 2 小时后，黏附在层粘连蛋白-磷灰石复合层上的细胞表现出明显更高的细胞保留率，而未经处理的 Ti、Ap 层和 AlbAp 复合层则没有。在生理剪切力下 2 小时后，L40Ap 上保留的细胞数量是未经处理的 Ti 的 9.5 倍。层粘连蛋白-磷灰石复合层是一种很有前途的用于血液接触材料内皮化的界面层。
