Uchida Masaki, Ito Atsuo, Furukawa Katsuko S, Nakamura Keigo, Onimura Yuji, Oyane Ayako, Ushida Takashi, Yamane Takashi, Tamaki Tamotsu, Tateishi Tetsuya
Institute for Human Science and Biomedical Engineering, National Institute of Advanced Industrial Science and Technology, Central 6, Tsukuba, Ibaraki, Japan.
Biomaterials. 2005 Dec;26(34):6924-31. doi: 10.1016/j.biomaterials.2005.04.066.
Titanium metal coated with apatite (HA-Ti), albumin-apatite composite (AA-Ti) or laminin-apatite composite (LA-Ti) was prepared by the immersion of NaOH- and heat-treated titanium metal in a calcium phosphate solution, or one supplemented with albumin or laminin. Platelet adhesion to the obtained materials under flow conditions was investigated in real time using a cone- and plate-type viscometer and fluorescence labeled platelets. Adhesion and activation of the platelets on the HA-Ti, AA-Ti and LA-Ti were definitely suppressed as compared with those on untreated titanium metal with a mirror surface. Furthermore, the numbers of platelets adhered to AA-Ti and LA-Ti are smaller than those adhered to HA-Ti, although the differences were not statistically significant. These findings suggest that HA-Ti, AA-Ti and LA-Ti, especially AA-Ti and LA-Ti, would exhibit thromboresistance that is superior to commercially pure titanium metal in terms of platelet adhesion.
通过将经氢氧化钠处理和热处理的钛金属浸入磷酸钙溶液或添加了白蛋白或层粘连蛋白的溶液中，制备了涂覆有磷灰石的钛金属（HA-Ti）、白蛋白-磷灰石复合材料（AA-Ti）或层粘连蛋白-磷灰石复合材料（LA-Ti）。使用锥板式粘度计和荧光标记的血小板实时研究了在流动条件下血小板对所得材料的粘附情况。与未经处理的镜面钛金属相比，HA-Ti、AA-Ti和LA-Ti上血小板的粘附和活化明显受到抑制。此外，尽管差异无统计学意义，但粘附在AA-Ti和LA-Ti上的血小板数量比粘附在HA-Ti上的少。这些发现表明，HA-Ti、AA-Ti和LA-Ti，尤其是AA-Ti和LA-Ti，在血小板粘附方面将表现出优于商业纯钛金属的抗血栓性。
