Mehrotra N K, Saxena A K
Br J Exp Pathol. 1979 Oct;60(5):518-25.
To evaluate the carcinogenic activity of jute-batching oil (JBO), this substance was painted on the skin of ITRC mice up to 300 days. Initially hyper- and parakeratosis of the stratum corneum, acanthosis and spongiosis of the stratum Malpighii, hyperactivity of fibroblasts, and laying down of collagen fibres in the dermis were encountered at 100 days. This was followed by poor hair growth, acne formation and ulceration. As time passed, these animals partially adapted themselves to the oil-painting so that by 200 days hyperkeratosis and parakeratosis of the stratum corneum, as well as acanthosis and spongiosis of the stratum Malpighii, had almost disappeared. The ulcers healed and no more acne was visible; however, the baldness and loss of hair appendages persisted to 300 days. No carcinogenic changes in the skin or in the viscera of these mice were observed. On UV and IR spectroscopy no traces of any polycyclic aromatic hydrocarbons were found in the JBO sample. Mice, on the other hand, when painted with the known carcinogen 3,4 benzpyrene (BP), developed skin tumours, showing that the mice used in this study were not cancer-resistant. Also, when JBO was applied with BP, the time taken for tumour development in mice was shortened by about 4 weeks as compared to another group painted with the same dose of BP alone. This suggests a cancer-promoting activity which needs to be investigated further.
为评估黄麻浸渍油（JBO）的致癌活性，将该物质涂抹在ITRC小鼠皮肤上长达300天。最初，在100天时观察到角质层的角化过度和不全角化、马尔皮基层的棘层肥厚和海绵形成、成纤维细胞活性增强以及真皮中胶原纤维的沉积。随后出现毛发生长不良、痤疮形成和溃疡。随着时间的推移，这些动物部分适应了涂抹油的情况，以至于到200天时，角质层的角化过度和不全角化以及马尔皮基层的棘层肥厚和海绵形成几乎消失。溃疡愈合，不再可见痤疮；然而，秃斑和毛发附属器缺失持续到300天。在这些小鼠的皮肤或内脏中未观察到致癌变化。在紫外和红外光谱分析中，在JBO样品中未发现任何多环芳烃的痕迹。另一方面，当用已知致癌物3,4-苯并芘（BP）涂抹小鼠时，会发生皮肤肿瘤，这表明本研究中使用的小鼠并非抗癌症的。此外，当将JBO与BP一起应用时，与另一组仅涂抹相同剂量BP的小鼠相比，小鼠发生肿瘤的时间缩短了约4周。这表明存在一种促癌活性，需要进一步研究。
