Agarwal R, Kumar S, Shukla Y, Antony M, Mehrotra N K
Cancer Lett. 1985 Sep 30;28(3):281-90. doi: 10.1016/0304-3835(85)90036-9.
In order to identify the tumour initiating constituent(s) of a mineral oil, jute batching oil (JBO), used in the processing of jute fibres, it was fractionally distilled in various boiling range fractions. The latter were then subjected to in vivo assessment of their aryl hydrocarbon hydroxylase (AHH) inducing potential in mouse epidermis. Fractions with almost similar AHH inducing potential were regrouped and studied for their tumour initiating potential over mouse skin following two-stage initiation-promotion protocol and using 12-O-tetradecanoyl phorbol-13-acetate (TPA) as tumour promoter. It was noticed that: (1) JBO as initiator, provoked local development of benign skin tumours over mouse back; (2) fractions of JBO boiling below 335 degrees C and above 399 degrees C accounted for most of the tumour initiating potential of the oil; (3) the histological features of the tumours (i.e. benign papillomas and keratoacanthomas) initiated by these fractions were similar to those developed after being initiated with unfractionated or reconstituted JBO; (4) removal of these fractions from JBO may be attempted which could decontaminate the batch oil from most of its tumorigenic components and make it safer for industrial use.
为了鉴定用于黄麻纤维加工的矿物油——黄麻成批油（JBO）中的肿瘤起始成分，将其分馏成不同沸程的馏分。然后对后者在小鼠表皮中的芳烃羟化酶（AHH）诱导潜力进行体内评估。将具有几乎相似AHH诱导潜力的馏分重新分组，并按照两阶段启动-促进方案，以12-O-十四烷酰佛波醇-13-乙酸酯（TPA）作为肿瘤促进剂，研究它们对小鼠皮肤的肿瘤起始潜力。结果发现：（1）JBO作为启动剂，引发了小鼠背部良性皮肤肿瘤的局部发展；（2）JBO沸程低于335℃和高于399℃的馏分占该油大部分的肿瘤起始潜力；（3）由这些馏分引发的肿瘤（即良性乳头状瘤和角化棘皮瘤）的组织学特征与用未分馏或重新配制的JBO启动后形成的肿瘤相似；（4）可以尝试从JBO中去除这些馏分，这可以从其大部分致瘤成分中净化该批油，并使其在工业使用中更安全。
