Isogenic autosomes to be applied in optimal screening for novel mutants with viable phenotypes in Drosophila melanogaster.

作者信息

Sharma Punita, Asztalos Zoltan, Ayyub Champakali, de Bruyne Marien, Dornan Anthony J, Gomez-Hernandez Araceli, Keane John, Killeen James, Kramer Susanne, Madhavan Mayur, Roe Helen, Sherkhane Pradeep Dagadu, Siddiqi Khalid, Silva Elizabeth, Carlson John R, Goodwin Stephen F, Heisenberg Martin, Krishnan Kits, Kyriacou Charalambos P, Partridge Linda, Riesgo-Escovar Juan, Rodrigues Veronica, Tully Tim, O'Kane Cahir J

机构信息

Department of Genetics, University of Cambridge, Cambridge, UK.

出版信息

J Neurogenet. 2005 Apr-Jun;19(2):57-85. doi: 10.1080/01677060591007155.

DOI:10.1080/01677060591007155

PMID:16024440

Abstract

Most insertional mutagenesis screens of Drosophila performed to date have not used target chromosomes that have been checked for their suitability for phenotypic screens for viable phenotypes. To address this, we have generated a selection of stocks carrying either isogenized second chromosomes or isogenized third chromosomes, in a genetic background derived from a Canton-S wild-type strain. We have tested these stocks for a range of behavioral and other viable phenotypes. As expected, most lines are statistically indistinguishable from Canton-S in most phenotypes tested. The lines generated are now being used as target chromosomes in mutagenesis screens, and the characterization reported here will facilitate their use in screens of these lines for behavioral and other viable phenotypes.

摘要