人类PAF复合物将转录与RNA合成下游的事件进行协调。
The human PAF complex coordinates transcription with events downstream of RNA synthesis.
作者信息
Zhu Bing, Mandal Subhrangsu S, Pham Anh-Dung, Zheng Yong, Erdjument-Bromage Hediye, Batra Surinder K, Tempst Paul, Reinberg Danny
机构信息
Howard Hughes Medical Institute, Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
出版信息
Genes Dev. 2005 Jul 15;19(14):1668-73. doi: 10.1101/gad.1292105.
The yeast PAF (yPAF) complex interacts with RNA polymerase II and coordinates the setting of histone marks associated with active transcription. We report the isolation and functional characterization of the human PAF (hPAF) complex. hPAF shares four subunits with yPAF (hCtr9, hPaf1, hLeo1, and hCdc73), but contains a novel higher eukaryotic-specific subunit, hSki8. RNAi against hSki8 or hCtr9 reduces the cellular levels of other hPAF subunits and of mono- and trimethylated H3-Lys 4 and dimethylated H3-Lys 79. The hSki8 subunit is also a component of the human SKI (hSKI) complex. Yeast SKI complex is cytoplasmic and together with Exosome mediates 3'-5' mRNA degradation. However, hSKI complex localizes to both nucleus and cytoplasm. Immunoprecipitation experiments revealed that hPAF and hSKI complexes interact, and ChIP experiments demonstrated that hSKI associates with transcriptionally active genes dependent on the presence of hPAF. Thus, in addition to coordinating events during transcription (initiation, promoter clearance, and elongation), hPAF also coordinates events in RNA quality control.
酵母PAF（yPAF）复合物与RNA聚合酶II相互作用，并协调与活跃转录相关的组蛋白标记的设定。我们报告了人类PAF（hPAF）复合物的分离和功能表征。hPAF与yPAF共享四个亚基（hCtr9、hPaf1、hLeo1和hCdc73），但包含一个新的高等真核生物特异性亚基hSki8。针对hSki8或hCtr9的RNA干扰会降低其他hPAF亚基以及单甲基化和三甲基化的H3-Lys 4和二甲基化的H3-Lys 79的细胞水平。hSki8亚基也是人类SKI（hSKI）复合物的一个组成部分。酵母SKI复合物位于细胞质中，并与外切体一起介导3'-5' mRNA降解。然而，hSKI复合物定位于细胞核和细胞质。免疫沉淀实验表明hPAF和hSKI复合物相互作用，染色质免疫沉淀实验表明hSKI与转录活跃基因相关，且这种相关性依赖于hPAF的存在。因此，除了在转录过程（起始、启动子清除和延伸）中协调相关事件外，hPAF还在RNA质量控制中协调相关事件。
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