Friedman Mendel, Lee Kap-Rang, Kim Hyun-Jeong, Lee In-Seon, Kozukue Nobuyuke
Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, California 94710, USA.
J Agric Food Chem. 2005 Jul 27;53(15):6162-9. doi: 10.1021/jf050620p.
Methods were devised for the isolation of large amounts of pure alpha-chaconine and alpha-solanine from Dejima potatoes and for the extraction and analysis of total glycoalkaloids from five fresh potato varieties (Dejima, Jowon, Sumi, Toya, and Vora Valley). These compounds were then evaluated in experiments using a tetrazolium microculture (MTT) assay to assess the anticarcinogenic effects of (a) the isolated pure glycoalkaloids separately, (b) artificial mixtures of the two glycoalkaloids, and (c) the total glycoalkaloids isolated from each of the five potato varieties. All samples tested reduced the numbers of the following human cell lines: cervical (HeLa), liver (HepG2), lymphoma (U937), stomach (AGS and KATO III) cancer cells and normal liver (Chang) cells. The results show that (a) the effects of the glycoalkaloids were concentration dependent in the range of 0.1-10 mug/mL (0.117-11.7 nmol/mL); (b) alpha-chaconine was more active than was alpha-solanine; (c) some mixtures exhibited synergistic effects, whereas other produced additive ones; (d) the different cancer cells varied in their susceptibilities to destruction; and (e) the destruction of normal liver cells was generally lower than that of cancer liver cells. The decreases in cell populations were also observed visually by reversed-phase microscopy. The results complement related observations on the anticarcinogenic potential of food ingredients.
已设计出从出岛土豆中分离大量纯α-查茄碱和α-茄碱的方法，以及从五个新鲜土豆品种（出岛、朝云、苏美、东亚和沃拉谷）中提取和分析总糖苷生物碱的方法。然后，在使用四唑盐微量培养（MTT）试验的实验中对这些化合物进行评估，以评估以下物质的抗癌作用：（a）单独分离出的纯糖苷生物碱；（b）两种糖苷生物碱的人工混合物；（c）从五个土豆品种中各自分离出的总糖苷生物碱。所有测试样品均减少了以下人类细胞系的数量：宫颈癌细胞（HeLa）、肝癌细胞（HepG2）、淋巴瘤细胞（U937）、胃癌细胞（AGS和KATO III）以及正常肝细胞（Chang）。结果表明：（a）糖苷生物碱的作用在0.1 - 10微克/毫升（0.117 - 11.7纳摩尔/毫升）范围内呈浓度依赖性；（b）α-查茄碱比α-茄碱更具活性；（c）一些混合物表现出协同作用，而其他混合物则产生相加作用；（d）不同癌细胞对破坏的敏感性各不相同；（e）正常肝细胞的破坏通常低于肝癌细胞。通过反相显微镜也能直观观察到细胞数量的减少。这些结果补充了关于食品成分抗癌潜力的相关观察。
