Vuu Kien, Xie Jianwu, McDonald Michael A, Bernardo Marcelino, Hunter Finie, Zhang Yantian, Li King, Bednarski Mark, Guccione Samira
Howard Hughes Medical Institute-National Institutes of Health Research Scholars Program, 4000 Jones Bridge Road, Chevy Chase, Maryland 20815-6789, USA.
Bioconjug Chem. 2005 Jul-Aug;16(4):995-9. doi: 10.1021/bc050085z.
A novel dual-labeled nanoparticle for use in labeling and tracking cells in vivo is described. We report the construction and characterization of these gadolinium-rhodamine nanoparticles. These particles are constructed from lipid monomers with diacetylene bonds that are sonicated and photolyzed to form polymerized nanoparticles. Cells are efficiently labeled with these nanoparticles. We have inoculated labeled tumor cells subcutaneouosly into the flanks of C3H mice and have been able to image these labeled tumor cells via MRI and optical imaging. Furthermore, the labeled tumor cells can be visualized via fluorescent microscopy after tissue biopsy. Our results suggest that these nanoparticles could be used to track cells in vivo. This basic platform can be modified with different fluorophores and targeting agents for studying metastisic cell, stem cell, and immune cell trafficking among other applications.
本文描述了一种用于体内细胞标记和追踪的新型双标记纳米颗粒。我们报告了这些钆 - 罗丹明纳米颗粒的构建及表征。这些颗粒由带有二乙炔键的脂质单体构建而成，经超声处理和光解形成聚合纳米颗粒。细胞能够被这些纳米颗粒有效标记。我们已将标记的肿瘤细胞皮下接种到C3H小鼠的胁腹，并能够通过磁共振成像（MRI）和光学成像对这些标记的肿瘤细胞进行成像。此外，在组织活检后，可通过荧光显微镜观察到标记的肿瘤细胞。我们的结果表明，这些纳米颗粒可用于体内追踪细胞。这个基础平台可以用不同的荧光团和靶向剂进行修饰，以用于研究转移细胞、干细胞和免疫细胞的迁移等其他应用。
