Kwon O-J, Hwang S-H, Heo Y-S, Hur S-S, Lee M-N, Oh H-B
BioSewoom Institute of Bioscience and Biotechnology, University of Ulsan College of Medicine, Seoul, Korea.
Tissue Antigens. 2005 Aug;66(2):141-4. doi: 10.1111/j.1399-0039.2005.00447.x.
In this report, we describe the identification of a human leucocyte antigen-A11 (HLA-A11) nucleotide sequence variant, a new HLA-A1120 by using sequence-based typing (SBT). The new allele was detected during routine HLA typing by high-resolution SBT. Allele A1120 showed one nucleotide difference with A110101 at codon 152 (GCG-->GAG) resulting in an amino acid change from alanine to glutamate. Residue 152 is located on alpha(2)-helix of HLA class I molecule and involved in peptide binding by constructing E pocket of peptide-binding groove, implying that the change of the residue 152 would affect the binding affinity of peptides to A1120 allele.
在本报告中，我们描述了通过基于序列的分型（SBT）鉴定出一种人类白细胞抗原-A11（HLA-A11）核苷酸序列变体，即一个新的HLA-A1120。该新等位基因是在通过高分辨率SBT进行常规HLA分型过程中检测到的。等位基因A1120在密码子152处与A110101存在一个核苷酸差异（GCG-->GAG），导致氨基酸从丙氨酸变为谷氨酸。第152位残基位于HLA I类分子的α(2)-螺旋上，通过构建肽结合槽的E口袋参与肽结合，这意味着第152位残基的改变会影响肽与A1120等位基因的结合亲和力。
