Kemna Erwin, Tjalsma Harold, Laarakkers Coby, Nemeth Elizabeta, Willems Hans, Swinkels Dorine
Department of Clinical Chemistry 564, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands.
Blood. 2005 Nov 1;106(9):3268-70. doi: 10.1182/blood-2005-05-1873. Epub 2005 Jul 19.
The hepatic peptide hormone hepcidin is the central regulator of iron metabolism and mediator of anemia of inflammation. To date, only one specific immuno-dot assay to measure hepcidin in urine had been documented. Here we report an alternative approach for quantification of hepcidin in urine by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Peptide peaks were detected corresponding to the 3 forms of hepcidin normally found in urine. The identity of the peptide peak equivalent to hepcidin-25 was confirmed using synthetic human hepcidin-25. Validation of our MS data on samples with various hepcidin levels showed a strong correlation with previous immuno-dot assay results (Spearman R = 0.9275, P < .001). Most importantly, this hepcidin assay clearly discriminates between relevant clinical iron disorders. In conclusion, this novel MS urine hepcidin assay is easy to perform and available to a wide audience. This enables the implementation of hepcidin measurements in large clinical studies.
肝脏肽激素铁调素是铁代谢的核心调节因子和炎症性贫血的介质。迄今为止，仅有一项用于测量尿液中铁调素的特异性免疫斑点测定法被记录在案。在此，我们报告一种通过表面增强激光解吸/电离飞行时间质谱法（SELDI-TOF-MS）对尿液中铁调素进行定量的替代方法。检测到与尿液中通常发现的3种铁调素形式相对应的肽峰。使用合成人铁调素-25确认了相当于铁调素-25的肽峰的身份。对具有不同铁调素水平的样本的质谱数据验证显示，与先前的免疫斑点测定结果具有很强的相关性（Spearman相关系数R = 0.9275，P <.001）。最重要的是，这种铁调素测定法能够清晰地区分相关的临床铁紊乱。总之，这种新型的尿液铁调素质谱测定法易于操作，可供广大用户使用。这使得在大型临床研究中开展铁调素测量成为可能。
