Schneider R, Schweiger M
Institut für Biochemie, Universität Innsbruck, Austria.
Mol Gen Genet. 1992 May;233(1-2):315-8. doi: 10.1007/BF00587594.
Mutations in the RNA1 gene of Saccharomyces cerevisiae, which encodes an essential cytosolic protein, affect the production and processing of all major classes of RNA. The mechanisms underlying these effects are not at all understood. Detailed comparative sequence analyses revealed that the RNA1 protein belongs to a superfamily, the members of which contain repetitive "leucine-rich motifs" (LRM). Within this superfamily RNA1 is most closely related to the ribonuclease/angiogenin inhibitor (RAI), which is a tightly binding inhibitor of ribonucleolytic activities in mammals. These results not only provide important clues to the structure, function and evolution of the RNA1 protein, but also have intriguing implications for possible novel functions of RAI.
酿酒酵母RNA1基因发生突变，该基因编码一种必需的胞质蛋白，会影响所有主要RNA类别的产生和加工。这些影响背后的机制完全不清楚。详细的比较序列分析表明，RNA1蛋白属于一个超家族，其成员包含重复的“富含亮氨酸基序”（LRM）。在这个超家族中，RNA1与核糖核酸酶/血管生成素抑制剂（RAI）关系最为密切，RAI是哺乳动物核糖核酸酶活性的紧密结合抑制剂。这些结果不仅为RNA1蛋白的结构、功能和进化提供了重要线索，也对RAI可能的新功能具有有趣的启示。
