Castorina C, Campisi A, Di Giacomo C, Sorrenti V, Russo A, Vanella A
Institute of Biochemistry, University of Catania, Italy.
Neurochem Res. 1992 Jun;17(6):599-604. doi: 10.1007/BF00968789.
In the present study, we have assayed the enzymatic activity of Cu,Zn-SOD, Mn-SOD, GSH-Px, GSH-Red, Cat, and G6PD in rat retina as a function of age. Conjugated diene levels and MDA formation were also determined. The conjugated diene levels in rat retina were found to increase significantly with age, accompanied by a marked decrease in GSH-Px and Cat activities. No age-related change in MDA levels and in GSH-Red and G6PD activity was found, whereas a significant increase in SOD activity was observed between 1 and 4 months. Decreased GSH-Px and Cat activity is related to increased lipid peroxidation with age.
在本研究中，我们测定了大鼠视网膜中铜锌超氧化物歧化酶（Cu,Zn-SOD）、锰超氧化物歧化酶（Mn-SOD）、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽还原酶（GSH-Red）、过氧化氢酶（Cat）和葡萄糖-6-磷酸脱氢酶（G6PD）的酶活性随年龄的变化情况。同时还测定了共轭二烯水平和丙二醛（MDA）的生成量。结果发现，大鼠视网膜中的共轭二烯水平随年龄显著增加，同时GSH-Px和Cat的活性显著降低。未发现MDA水平以及GSH-Red和G6PD活性随年龄有变化，而在1至4个月之间观察到超氧化物歧化酶（SOD）活性显著增加。GSH-Px和Cat活性降低与年龄增长导致的脂质过氧化增加有关。
