Malherbe Y, Kamping A, van Delden W, van de Zande L
Evolutionary Genetics, Centre for Ecological and Evolutionary Studies, Biological Centre, University of Groningen, The Netherlands.
J Evol Biol. 2005 Jul;18(4):811-9. doi: 10.1111/j.1420-9101.2004.00877.x.
In Drosophila melanogaster, alcohol dehydrogenase (ADH) activity is essential for ethanol tolerance, but its role may not be restricted to alcohol metabolism alone. Here we describe ADH activity and Adh expression level upon selection for increased alcohol tolerance in different life-stages of D. melanogaster lines with two distinct Adh genotypes: Adh(FF) and Adh(SS). We demonstrate a positive within genotype response for increased alcohol tolerance. Life-stage dependent selection was observed in larvae only. A slight constitutive increase in adult ADH activity for all selection regimes and genotypes was observed, that was not paralleled by Adh expression. Larval Adh expression showed a constitutive increase, that was not reflected in ADH activity. Upon exposure to environmental ethanol, sex, selection regime life stage and genotype appear to have differential effects. Increased ADH activity accompanies increased ethanol tolerance in D. melanogaster but this increase is not paralleled by expression of the Adh gene.
在黑腹果蝇中，乙醇脱氢酶（ADH）活性对于乙醇耐受性至关重要，但其作用可能并不局限于酒精代谢。在这里，我们描述了在具有两种不同Adh基因型（Adh(FF)和Adh(SS)）的黑腹果蝇品系的不同生命阶段中，选择提高酒精耐受性后ADH活性和Adh表达水平的情况。我们证明了在基因型内对提高酒精耐受性存在正向反应。仅在幼虫中观察到了依赖生命阶段的选择。在所有选择方案和基因型中，成虫的ADH活性都有轻微的组成性增加，但Adh表达并未与之平行。幼虫的Adh表达有组成性增加，但这并未在ADH活性中体现出来。暴露于环境乙醇后，性别、选择方案、生命阶段和基因型似乎有不同的影响。黑腹果蝇中ADH活性增加伴随着乙醇耐受性增加，但这种增加与Adh基因的表达并不平行。
