猫冠状病毒毒株FIPV WSU-79/1146的基因组RNA序列。
Genomic RNA sequence of Feline coronavirus strain FIPV WSU-79/1146.
作者信息
Dye Charlotte, Siddell Stuart G
机构信息
Division of Virology, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.
出版信息
J Gen Virol. 2005 Aug;86(Pt 8):2249-2253. doi: 10.1099/vir.0.80985-0.
Abstract
A consensus sequence of the Feline coronavirus (FCoV) (strain FIPV WSU-79/1146) genome was determined from overlapping cDNA fragments produced by RT-PCR amplification of viral RNA. The genome was found to be 29 125 nt in length, excluding the poly(A) tail. Analysis of the sequence identified conserved open reading frames and revealed an overall genome organization similar to that of other coronaviruses. The genomic RNA was analysed for putative cis-acting elements and the pattern of subgenomic mRNA synthesis was analysed by Northern blotting. Comparative sequence analysis of the predicted FCoV proteins identified 16 replicase proteins (nsp1-nsp16) and four structural proteins (spike, membrane, envelope and nucleocapsid). Two mRNAs encoding putative accessory proteins were also detected. Phylogenetic analyses confirmed that FIPV WSU-79/1146 belongs to the coronavirus subgroup G1-1. These results confirm and extend previous findings from partial sequence analysis of FCoV genomes.
摘要
通过对猫冠状病毒（FCoV）（毒株FIPV WSU-79/1146）病毒RNA进行RT-PCR扩增产生的重叠cDNA片段，确定了其基因组的共有序列。发现该基因组长度为29125个核苷酸，不包括聚腺苷酸尾。对该序列的分析确定了保守的开放阅读框，并揭示了与其他冠状病毒相似的整体基因组结构。对基因组RNA进行了推定顺式作用元件分析，并通过Northern印迹分析了亚基因组mRNA的合成模式。对预测的FCoV蛋白进行比较序列分析，确定了16种复制酶蛋白（nsp1-nsp16）和4种结构蛋白（刺突蛋白、膜蛋白、包膜蛋白和核衣壳蛋白）。还检测到了两种编码推定辅助蛋白的mRNA。系统发育分析证实FIPV WSU-79/1146属于冠状病毒G1-1亚组。这些结果证实并扩展了先前对FCoV基因组部分序列分析的结果。
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