Sebeková Katarína, Hofmann Thomas, Boor Peter, Sebeková Katarína, Ulicná Ol'ga, Erbersdobler Helmut F, Baynes John W, Thorpe Suzanne R, Heidland August, Somoza Veronika
Slovak Medical University, Institute of Preventive and Clinical Medicine, Limbová 14, 833 03 Bratislava, Slovakia.
Ann N Y Acad Sci. 2005 Jun;1043:482-91. doi: 10.1196/annals.1333.055.
The biological consequences of chronic consumption of Maillard reaction products (MRPs) on renal function in health and renal disease are still incompletely understood. We investigated the metabolic and renal effects of a diet with varying MRP content in healthy and subtotally nephrectomized rats. Male Wistar rats were subjected to sham operation (control, C, n = 12), or to 5/6 nephrectomy (5/6NX, n = 12). Both groups were randomized into subgroups and pair-fed with either a MRP-poor or -rich diet for six weeks. The diet was prepared by replacing 5% or 25% of wheat starch by bread crust (BC). In spite of pair-feeding, the rats on the 25% BC diet gained more body weight (C: 183 +/- 6 g; C + 5% BC: 197 +/- 7 g; C + 25% BC: 229 +/- 6 g [P < 0.05]; 5/6NX: 165 +/- 10 g; 5/6NX + 5% BC: 202 +/- 3 g; 5/6NX + 25% BC: 209 +/- 8 g [P < 0.05]) and had a higher organ weight (heart, liver, lung, kidney/remnant kidney). Bread crust-enriched diet induced proteinuria (C: 15 +/- 5 mg/24 h; C + 5% BC: 19 +/- 4; C + 25% BC: 26 +/- 3 [P < 0.05]; 5/6NX: 30 +/- 7 mg/24 h; 5/6NX + 5% BC: 47 +/- 9; 5/6NX + 25% BC: 87 +/- 19 [P < 0.01]) and a rise in urinary transforming growth factor beta(1) excretion (C: 0.4 +/- 0.1 ng/24 h; C + 5% BC: 0.6 +/- 0.1; C + 25% BC: 1.2 +/- 0.3; 5/6NX: 0.5 +/- 0.1 ng/24 h; 5/6NX + 5% BC: 0.9 +/- 0.1; 5/6NX + 25% BC: 1.6 +/- 0.2 [P < 0.01]). Plasma creatinine or creatinine clearance were not affected significantly. In conclusion, our data suggests that long-term consumption of a diet rich in MRPs may lead to damage of the kidneys.
长期食用美拉德反应产物（MRP）对健康和肾脏疾病患者肾功能的生物学影响仍未完全明确。我们研究了不同MRP含量饮食对健康和次全肾切除大鼠的代谢及肾脏影响。雄性Wistar大鼠接受假手术（对照组，C，n = 12）或5/6肾切除术（5/6NX，n = 12）。两组均随机分为亚组，分别用低MRP或高MRP饮食配对喂养六周。饮食通过用面包皮（BC）替代5%或25%的小麦淀粉来制备。尽管采用了配对喂养，但食用25% BC饮食的大鼠体重增加更多（C组：183±6 g；C + 5% BC组：197±7 g；C + 25% BC组：229±6 g [P < 0.05]；5/6NX组：165±10 g；5/6NX + 5% BC组：202±3 g；5/6NX + 25% BC组：209±8 g [P < 0.05]），且器官重量更高（心脏、肝脏、肺、肾脏/残余肾脏）。富含面包皮的饮食导致蛋白尿（C组：15±5 mg/24 h；C + 5% BC组：19±4 mg/24 h；C + 25% BC组：26±3 mg/24 h [P < 0.05]；5/6NX组：30±7 mg/24 h；5/6NX + 5% BC组：47±9 mg/24 h；5/6NX + 25% BC组：87±19 mg/24 h [P < 0.01]）以及尿转化生长因子β(1)排泄增加（C组：0.4±0.1 ng/24 h；C + 5% BC组：0.6±0.1 ng/24 h；C + 25% BC组：1.2±0.3 ng/24 h；5/6NX组：0.5±0.1 ng/24 h；5/6NX + 5% BC组：0.9±0.1 ng/24 h；5/6NX + 25% BC组：1.6±0.2 ng/24 h [P < 0.01]）。血浆肌酐或肌酐清除率未受到显著影响。总之，我们的数据表明长期食用富含MRP的饮食可能导致肾脏损伤。
