Avunduk C, Eastwood G L, Polakowski N, Burstein S
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655.
Prostaglandins Leukot Essent Fatty Acids. 1992 Apr;45(4):329-32. doi: 10.1016/0952-3278(92)90091-v.
To determine the effect of different doses of hydrocortisone sodium succinate (HC) on rat gastric mucosal prostaglandin synthesis, two experiments were performed. In the first experiment, 20 male Lewis rats were divided into 4 groups of 5 rats each and gavaged either with 2 ml of water (control) or different concentrations of HC (10 mg/ml, 100 mg/ml and 500 mg/ml). In the second experiment in a similar design, lower doses of HC were used (water, 0.1 mg/ml, 0.50 mg/ml and 5.0 mg/ml). The rats were killed after 1 h and three 3 x 3 mm pieces of gastric tissue were removed from each rat and incubated for the determination of prostaglandin E2 and 6-keto-prostaglandin F1 alpha accumulation in the medium measured by radioimmunoassay. At low doses HC inhibits rat gastric mucosal prostaglandin synthesis whereas at higher doses HC stimulates it. This biphasic effect of HC on gastric mucosal prostaglandin synthesis may help explain its role in ulcerogenesis.
为确定不同剂量的氢化可的松琥珀酸钠（HC）对大鼠胃黏膜前列腺素合成的影响，进行了两项实验。在第一项实验中，将20只雄性Lewis大鼠分为4组，每组5只，分别灌胃2毫升水（对照组）或不同浓度的HC（10毫克/毫升、100毫克/毫升和500毫克/毫升）。在第二项实验中，采用类似设计，使用了较低剂量的HC（水、0.1毫克/毫升、0.50毫克/毫升和5.0毫克/毫升）。1小时后处死大鼠，从每只大鼠身上取出3块3×3毫米的胃组织块，进行孵育，以通过放射免疫测定法测定培养基中前列腺素E2和6-酮-前列腺素F1α的积累量。低剂量时HC抑制大鼠胃黏膜前列腺素合成，而高剂量时HC则刺激其合成。HC对胃黏膜前列腺素合成的这种双相作用可能有助于解释其在溃疡形成中的作用。
