Eastwood G L, Avunduk C, Quimby G F
Medical College of Georgia, Augusta.
Dig Dis Sci. 1993 Aug;38(8):1450-2. doi: 10.1007/BF01308602.
Previous studies have shown that cigarette smoking depresses prostaglandin generation by human gastric mucosa, but the component of smoke that is responsible for that action is not known. To investigate whether nicotine has a direct effect on gastric mucosal prostaglandin generation, we performed the following study. Eight rats were sacrificed and the stomachs removed. Using a biopsy forceps, small pieces of gastric mucosa were resected and placed in incubation vials containing either buffered Krebs solution alone (control), Krebs solution plus indomethacin (5 micrograms/ml), or Krebs solution plus one of several concentrations of nicotine ditartrate (10, 100, 500, 1000 ng/ml). The nicotine concentrations we used ranged below and above the plasma nicotine concentrations of smokers shortly after smoking cigarettes. Three separate incubations of gastric mucosa were performed per experimental group from each animal. After 30 min of incubation, prostaglandin E2 and 6-keto-prostaglandin F1 alpha concentrations in the incubation medium were measured by radioimmunoassay. We found that nicotine at any concentration tested had no effect on the generation of prostaglandin E2 and 6-keto-prostaglandin F1 alpha by rat gastric mucosa. Thus, this study indicates that, if nicotine is involved in the depression of prostaglandin generation in the gastric mucosa of smokers, its role is an indirect one and not by direct action on the gastric mucosa.
先前的研究表明，吸烟会抑制人胃黏膜中前列腺素的生成，但导致这种作用的烟雾成分尚不清楚。为了研究尼古丁是否对胃黏膜前列腺素的生成有直接影响，我们进行了以下研究。处死8只大鼠并取出胃。用活检钳切除小块胃黏膜并置于含有单独的缓冲克雷布斯溶液（对照）、克雷布斯溶液加吲哚美辛（5微克/毫升）或克雷布斯溶液加几种浓度的酒石酸二尼古丁（10、100、500、1000纳克/毫升）之一的培养瓶中。我们使用的尼古丁浓度低于和高于吸烟者吸烟后不久的血浆尼古丁浓度。对每只动物的每个实验组进行三次单独的胃黏膜培养。培养30分钟后，通过放射免疫测定法测量培养基中前列腺素E2和6-酮-前列腺素F1α的浓度。我们发现，所测试的任何浓度的尼古丁对大鼠胃黏膜中前列腺素E2和6-酮-前列腺素F1α的生成均无影响。因此，本研究表明，如果尼古丁参与吸烟者胃黏膜中前列腺素生成的抑制作用，其作用是间接的，而非直接作用于胃黏膜。
