功能性蛋白质定向进化的新基因型-表型联系。
New genotype-phenotype linkages for directed evolution of functional proteins.
作者信息
Leemhuis Hans, Stein Viktor, Griffiths Andrew D, Hollfelder Florian
机构信息
Department of Biochemistry, Cambridge University, Cambridge CB2 1GA, UK.
出版信息
Curr Opin Struct Biol. 2005 Aug;15(4):472-8. doi: 10.1016/j.sbi.2005.07.006.
The key practical consideration in directed evolution of functional biomolecules is the linkage of genotype and phenotype. In vitro selections offer the potential to select from libraries with up to 10(10)-10(14) members, with fewer constraints than current cell-based selections. New approaches such as mRNA display, ribosome display and in vitro compartmentalisation have complementary areas of application in selections for binding or catalysis.
在功能性生物分子的定向进化中，关键的实际考虑因素是基因型与表型的联系。体外筛选提供了从成员多达10(10)-10(14)的文库中进行选择的潜力，其限制比当前基于细胞的筛选更少。诸如mRNA展示、核糖体展示和体外区室化等新方法在结合或催化选择中具有互补的应用领域。
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