Yan Xianghua, Xu Zirong
Antibody Engineering Center, Key Laboratory of Animal Molecular Nutrition, Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou, 310029, P.R. China.
Drug Discov Today. 2006 Oct;11(19-20):911-6. doi: 10.1016/j.drudis.2006.08.012. Epub 2006 Sep 7.
In vitro display technologies, especially ribosome display, are valuable tools for many applications. In this paper, ribosome display technology and its applications for directed evolution of functional proteins will be reviewed. Ribosome display has great potential for directed evolution of protein stability and affinity, the generation of high-quality libraries by in vitro preselection, the selection of enzymatic activities, and the display of cDNA and random-peptide libraries. Ribosome display is carried out fully in vitro, which overcomes some of the limitations of cell-based display systems. We anticipate that ribosome display will have a great impact on applications in biotechnology, medicine and proteomics.
体外展示技术，尤其是核糖体展示技术，是适用于多种应用的宝贵工具。本文将对核糖体展示技术及其在功能性蛋白质定向进化中的应用进行综述。核糖体展示技术在蛋白质稳定性和亲和力的定向进化、通过体外预筛选生成高质量文库、酶活性的筛选以及cDNA文库和随机肽文库的展示方面具有巨大潜力。核糖体展示完全在体外进行，克服了基于细胞的展示系统的一些局限性。我们预计核糖体展示技术将对生物技术、医学和蛋白质组学的应用产生重大影响。
