Adojaan Maarja, Kivisild Toomas, Männik Andres, Krispin Tõnu, Ustina Valentina, Zilmer Kai, Liebert Elo, Jaroslavtsev Nikolai, Priimägi Ludmilla, Tefanova Valentina, Schmidt Jelena, Krohn Kai, Villems Richard, Salminen Mika, Ustav Mart
FIT Biotech Oyj Plc Eesti, Tartu, Estonia.
J Acquir Immune Defic Syndr. 2005 Aug 15;39(5):598-605.
An earlier study has indicated that a complex recombinant HIV-1 strain dominates the epidemic in Estonia. The objective of this study was to further investigate the molecular epidemiology and genetic structure of HIV-1 in Estonia. Most of the investigated individuals became infected after August 2000 when HIV-1 started to spread rapidly among Estonian intravenous drug users (IDUs). Two viral DNA regions, gag/pol and gp41, were sequenced and subtyped from peripheral blood mononuclear cells or plasma from 141 individuals. Phylogenetic analysis in the gp41 region revealed that the most frequent type of the virus among IDUs was a circulating recombinant form, CRF06_cpx, whereas a few samples showed highest sequence similarity to a subtype A strain circulating in Ukraine and Russia. Likewise, in the gag/pol region, most of the samples were classified as CRF06_cpx, with a few classified as subtype A. In this region, however, 16% of the sequences turned out to be mosaic unique recombinant forms consisting of CRF06_cpx and subtype A. At least 9 mosaic forms were identified, each with distinct patterns of multiple crossover. To characterize Estonian CRF06_cpx as well as recombinant isolates in more detail, 4 near-full-length HIV-1 genomes were sequenced.
一项早期研究表明，一种复杂的重组HIV-1毒株在爱沙尼亚的艾滋病流行中占据主导地位。本研究的目的是进一步调查爱沙尼亚HIV-1的分子流行病学和基因结构。大多数被调查个体在2000年8月之后感染，当时HIV-1开始在爱沙尼亚静脉注射吸毒者（IDU）中迅速传播。对141名个体外周血单核细胞或血浆中的两个病毒DNA区域gag/pol和gp41进行了测序和亚型分析。gp41区域的系统发育分析显示，IDU中最常见的病毒类型是一种循环重组形式CRF06_cpx，而少数样本与在乌克兰和俄罗斯流行的A亚型毒株序列相似性最高。同样，在gag/pol区域，大多数样本被归类为CRF06_cpx，少数被归类为A亚型。然而，在该区域，16%的序列被证明是由CRF06_cpx和A亚型组成的镶嵌独特重组形式。至少鉴定出9种镶嵌形式，每种都有不同的多重交叉模式。为了更详细地描述爱沙尼亚的CRF06_cpx以及重组分离株，对4个接近全长的HIV-1基因组进行了测序。
