Henderson Laura, Bond David, Simpson Trefford
University of Waterloo, School of Optometry, Waterloo, Ontario, Canada.
Optom Vis Sci. 2005 Jul;82(7):629-32. doi: 10.1097/01.opx.0000171817.32551.05.
The purpose of this study is to determine the association between corneal sensitivity measured using a pneumatic esthesiometer and eye color quantified objectively.
Twenty subjects had ocular surface sensitivity measured using a Belmonte esthesiometer. An ascending method of limits followed by the method of constant stimuli were used to estimate 1) cold detection thresholds, 2) discomfort detection thresholds (both using pneumatic stimuli at 20 degrees C, 3) mechanical detection thresholds using pneumatic stimuli at 50 degrees C (ocular surface temperature approximately 33 degrees C), and 4) percent CO2 chemical detection thresholds using 50 degrees C pneumatic stimuli at flow rates set at half of each subject's pneumatic detection threshold (therefore detected by the chemical content and not the mechanical content). Eye color was estimated 1) clinically by two observers ranking the color (light to dark) of digital images of each subject's iris, 2) photometrically by measuring iris luminance, and 3) using chromaticity obtained from a Photo Research 650 spectroradiometer with controlled illumination. Correlation and linear and nonlinear regression analyses were used to examine relationships between variables.
There were no associations between eye color (determined clinically or objectively) for mechanical and chemical detection thresholds (best r = 0.15, all p > 0.05). There was a significant linear association between 20 degrees detection thresholds and eye color (r = 0.39), which was substantially improved with a two-line function (part level and part increasing linearly, r = 0.65).
We were generally unable to demonstrate the relationship between eye color and sensitivity reported previously using a Cochet-Bonnet esthesiometer. However, for a subset of subjects with palest irises, there appears to be a linear association between eye color and sensitivity to cooling stimuli.
本研究旨在确定使用气动式触觉计测量的角膜敏感性与客观量化的眼睛颜色之间的关联。
20名受试者使用贝尔蒙特触觉计测量眼表敏感性。采用极限递增法，随后使用恒定刺激法来估计：1）冷觉检测阈值；2）不适检测阈值（均使用20摄氏度的气动刺激）；3）使用50摄氏度的气动刺激（眼表温度约为33摄氏度）的机械检测阈值；4）使用50摄氏度的气动刺激，流速设定为每个受试者气动检测阈值的一半时的二氧化碳化学检测阈值（因此是由化学成分而非机械成分检测到）。眼睛颜色通过以下方式估计：1）由两名观察者对每个受试者虹膜的数字图像颜色（从浅到深）进行临床排序；2）通过测量虹膜亮度进行光度测量；3）使用配备可控照明的Photo Research 650分光辐射计获得的色度。使用相关性分析以及线性和非线性回归分析来检验变量之间的关系。
机械和化学检测阈值的眼睛颜色（通过临床或客观确定）之间无关联（最佳r = 0.15，所有p>0.05）。20摄氏度检测阈值与眼睛颜色之间存在显著的线性关联（r = 0.39），使用二线函数（部分水平和部分线性增加，r = 0.65）时这种关联得到显著改善。
我们通常无法证明先前使用科谢 - 博内触觉计所报道的眼睛颜色与敏感性之间的关系。然而，对于虹膜最浅的一部分受试者，眼睛颜色与对冷却刺激的敏感性之间似乎存在线性关联。
