von Bohlen Und Halbach Oliver, Hinz Ulla, Unsicker Klaus, Egorov Alexei V
Interdisciplinary Center for Neurosciences, Department of Neuroanatomy, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany.
Cell Tissue Res. 2005 Nov;322(2):201-6. doi: 10.1007/s00441-005-0004-4. Epub 2005 Nov 3.
The transient receptor potential (TRP) superfamily comprises a group of non-selective cation channels that have been implicated in both receptor and store-operated channel functions. The family of the classical TRPs (TRPCs) consists of seven members (TRPC1-7). The presence of TRPC1 and TRPC5 mRNA in the brain has previously been demonstrated by real-time polymerase chain reaction. However, the distribution of these receptors within different brain areas of mice has not been investigated in detail. We have used antibodies directed against TRPC1 and TRPC5 to study the distribution and localization of these channels in murine medial temporal lobe structures. Both TRPC1 and TRPC5 channels are present in the various nuclei of the amygdala, in the hippocampus, and in the subiculum and the entorhinal cortex. We have found that TRPC1 channels are primarily expressed on cell somata and on dendrites, whereas TRPC5 channels are exclusively located on cell bodies. Moreover, TRPC1 channels are selectively expressed by neurons, whereas TRPC5 channels are mainly expressed by neurons, but also by non-neuronal cells. The expression of TRPC1 and TRPC5 channels in mammalian temporal lobe structures suggests their involvement in neuronal plasticity, learning and memory.
瞬时受体电位（TRP）超家族由一组非选择性阳离子通道组成，这些通道与受体功能和储存操纵性通道功能均有关联。经典TRP（TRPC）家族由七个成员（TRPC1 - 7）组成。先前通过实时聚合酶链反应已证实大脑中存在TRPC1和TRPC5 mRNA。然而，尚未对这些受体在小鼠不同脑区的分布进行详细研究。我们使用针对TRPC1和TRPC5的抗体来研究这些通道在小鼠内侧颞叶结构中的分布和定位。TRPC1和TRPC5通道均存在于杏仁核的各个核团、海马体、下托和内嗅皮质中。我们发现，TRPC1通道主要表达于细胞体和树突上，而TRPC5通道仅位于细胞体上。此外，TRPC1通道由神经元选择性表达，而TRPC5通道主要由神经元表达，但也由非神经元细胞表达。TRPC1和TRPC5通道在哺乳动物颞叶结构中的表达表明它们参与了神经元可塑性、学习和记忆过程。
