De March Zena, Giampà Carmela, Patassini Stefano, Bernardi Giorgio, Fusco Francesca R
Santa Lucia Foundation IRCCS Hospital at the European Centre for Brain Research, University of Rome Tor Vergata, Department of Neuroscience, Italy.
Neurosci Lett. 2006 Jul 10;402(1-2):35-9. doi: 10.1016/j.neulet.2006.03.061. Epub 2006 Apr 25.
Transient receptor potential channels (TRPC) are plasma membrane, non-selective cationic channels and have been proposed as candidates involved in the regulation of cellular Ca2+ influx. TRPC are involved in metabotropic glutamate receptor (mGluR)-mediated excitatory post-synaptic currents (EPSCs) in the dopaminergic neurons of the substantia nigra. We previously observed several subtypes of TRPC to be expressed at an mRNA level in the substantia nigra dopamine neurons. In particular, TRPC1 and TRPC5 are most frequently expressed in the substantia nigra. Our recent immunohistochemical findings showed that TRPC1 are mainly distributed in the dendrites of dopamine neurons. In the present study we have investigated, by means of immunohistochemistry and dual label immunofluorescence, the anatomical distribution of TRPC5 in the substantia nigra, and we have shown their preferential localization into the neuronal nuclei. Our findings suggest a role of TRPs in the calcium signaling system of the nucleus, although its physiological meaning needs further investigations.
瞬时受体电位通道（TRPC）是质膜上的非选择性阳离子通道，被认为是参与调节细胞钙内流的候选通道。TRPC参与黑质多巴胺能神经元中代谢型谷氨酸受体（mGluR）介导的兴奋性突触后电流（EPSC）。我们之前观察到几种TRPC亚型在黑质多巴胺神经元的mRNA水平表达。特别是，TRPC1和TRPC5在黑质中表达最为频繁。我们最近的免疫组织化学研究结果表明，TRPC1主要分布在多巴胺神经元的树突中。在本研究中，我们通过免疫组织化学和双标免疫荧光法研究了TRPC5在黑质中的解剖分布，并表明它们优先定位于神经元细胞核。我们的研究结果表明瞬时受体电位通道在细胞核的钙信号系统中发挥作用，尽管其生理意义需要进一步研究。
