Kong Lijun, Gao Yuan, Cao Wenling, Gong Yandao, Zhao Nanming, Zhang Xiufang
Department of Biological Sciences and Biotechnology, State Key Lab of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China.
J Biomed Mater Res A. 2005 Nov 1;75(2):275-82. doi: 10.1002/jbm.a.30414.
A novel nano-hydroxyapatite (HA)/chitosan composite scaffold with high porosity was developed. The nano-HA particles were made in situ through a chemical method and dispersed well on the porous scaffold. They bound to the chitosan scaffolds very well. This method prevents the migration of nano-HA particles into surrounding tissues to a certain extent. The morphologies, components, and biocompatibility of the composite scaffolds were investigated. Scanning electron microscopy, porosity measurement, thermogravimetric analysis, X-ray diffraction, X-ray photoelectron spectroscopy, and Fourier transformed infrared spectroscopy were used to analyze the physical and chemical properties of the composite scaffolds. The biocompatibility was assessed by examining the proliferation and morphology of MC 3T3-E1 cells seeded on the scaffolds. The composite scaffolds showed better biocompatibility than pure chitosan scaffolds. The results suggest that the newly developed nano-HA/chitosan composite scaffolds may serve as a good three-dimensional substrate for cell attachment and migration in bone tissue engineering.
开发了一种具有高孔隙率的新型纳米羟基磷灰石（HA）/壳聚糖复合支架。纳米HA颗粒通过化学方法原位制备，并很好地分散在多孔支架上。它们与壳聚糖支架结合良好。该方法在一定程度上防止了纳米HA颗粒向周围组织的迁移。研究了复合支架的形态、成分和生物相容性。使用扫描电子显微镜、孔隙率测量、热重分析、X射线衍射、X射线光电子能谱和傅里叶变换红外光谱来分析复合支架的物理和化学性质。通过检查接种在支架上的MC 3T3-E1细胞的增殖和形态来评估生物相容性。复合支架显示出比纯壳聚糖支架更好的生物相容性。结果表明，新开发的纳米HA/壳聚糖复合支架可作为骨组织工程中细胞附着和迁移的良好三维基质。
