Endodontic Department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
Human Anatomy and Embryology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
BMC Oral Health. 2023 May 1;23(1):252. doi: 10.1186/s12903-023-02975-3.
To study the odontogenic potential of dental pulp stem cells (DPSCs) after induction with three different bioactive materials: activa bioactive (base/liner) (AB), TheraCal LC (TC), and mineral trioxide aggregate (MTA), when combined with two different types of scaffolds.
DPSCs were isolated from freshly extracted premolars of young orthodontic patients, cultured, expanded to passage 4 (P), and characterized by flow cytometric analysis. DPSCs were seeded onto two scaffolds in contact with different materials (AB, TC, and MTA). The first scaffold contained polycaprolactone-nano-chitosan and synthetic hydroxyapatite (PCL-NC-HA), whereas the second scaffold contained polycaprolactone-nano-chitosan and synthetic Mg-substituted hydroxyapatite (PCL-NC-Mg-HA). DPSC viability and proliferation were evaluated at various time points. To assess odontoblastic differentiation, gene expression analysis of dentin sialophosphoprotein (DSPP) by quantitative real-time polymerase chain reaction (qRT-PCR) and morphological changes in cells were performed using inverted microscope phase contrast images and scanning electron microscopy. The fold-change in DSPP between subgroups was compared using a one-way ANOVA. Tukey's test was used to compare the fold-change in DSPP between the two subgroups in multiple comparisons, and P was set at p < 0.05.
DSPP expression was significantly higher in the PCL-NC-Mg-HA group than in the PCL-NC-HA group, and scanning electron microscopy revealed a strong attachment of odontoblast-like cells to the scaffold that had a stronger odontogenic differentiation effect on DPSCs than the scaffold that did not contain magnesium. MTA has a significantly higher odontogenic differentiation effect on cultured DPSCs than AB or TC does. The combination of scaffolds and bioactive materials improves DPSCs induction in odontoblast-like cells.
The PCL-NC-Mg-HA scaffold showed better odontogenic differentiation effects on cultured DPSCs. Compared to AB and TC, MTA is the most effective bioactive material for inducing the odontogenic differentiation of cultured DPSCs.
研究牙髓干细胞（DPSCs）在三种不同生物活性材料（活髓诱导剂基础/衬层（AB）、TheraCal LC（TC）和矿物三氧化物聚合体（MTA））诱导下与两种不同支架结合后的成牙本质潜能。
从年轻正畸患者的新鲜拔除前磨牙中分离 DPSCs，培养、扩增至第 4 代（P），并通过流式细胞术分析进行鉴定。将 DPSCs 接种到与不同材料（AB、TC 和 MTA）接触的两种支架上。第一种支架含有聚己内酯-纳米壳聚糖和合成羟基磷灰石（PCL-NC-HA），而第二种支架含有聚己内酯-纳米壳聚糖和合成 Mg 取代羟基磷灰石（PCL-NC-Mg-HA）。在不同时间点评估 DPSCs 的活力和增殖。为了评估成牙本质分化，通过实时定量聚合酶链反应（qRT-PCR）对牙本质涎磷蛋白（DSPP）的基因表达进行分析，并通过倒置显微镜相差图像和扫描电子显微镜观察细胞形态变化。采用单因素方差分析比较亚组之间 DSPP 的倍数变化。采用 Tukey 检验进行多重比较中两个亚组之间 DSPP 的倍数变化比较，p 值设为 p<0.05。
PCL-NC-Mg-HA 组的 DSPP 表达明显高于 PCL-NC-HA 组，扫描电镜显示，与不含镁的支架相比，具有更强成牙本质分化作用的支架上有更强附着的成牙本质样细胞。MTA 对培养的 DPSCs 的成牙本质分化作用明显高于 AB 或 TC。支架和生物活性材料的组合可提高 DPSCs 向成牙本质样细胞的诱导。
PCL-NC-Mg-HA 支架对培养的 DPSCs 显示出更好的成牙本质分化作用。与 AB 和 TC 相比，MTA 是诱导培养的 DPSCs 成牙本质分化最有效的生物活性材料。
